GALNT10 antibody (AA 151-250) (HRP)

Catalog No. ABIN1711826

The Rabbit Polyclonal anti-GALNT10 antibody is suitable to detect GALNT10 in samples from Human. It has been validated for WB, ELISA, IHC (p) and IHC (fro).

Quick Overview for GALNT10 antibody (AA 151-250) (HRP) (ABIN1711826)

Target: GALNT10 (UDP-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 10 (GalNAc-T10) (GALNT10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GALNT10 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GALNT10 Antibody (HRP)

Binding Specificity: AA 151-250

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GALNT10/GalNAc-T10

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GALNT10

Target: GALNT10 (UDP-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 10 (GalNAc-T10) (GALNT10))

Alternative Name: GALNT10/GalNAc-T10

Background:

Synonyms: DKFZp586H0623, FLJ00205, FLJ11715, GalNAc T10, GalNAc transferase 10, GalNAc-T10, GalNAcT10, GALNT10, GLT10_HUMAN, Polypeptide GalNAc transferase 10, Polypeptide N-acetylgalactosaminyltransferase 10, pp GalNAc T10, pp GaNTase 10, pp-GaNTase 10, ppGalNAcT10, ppGaNTase 10, Protein UDP acetylgalactosaminyltransferase 10, Protein-UDP acetylgalactosaminyltransferase 10, UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 10, UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 10, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10.

Background: The UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes are substrate-specific proteins that catalyze the transfer of GalNAc (N-acetylgalactosaminyl) to serine and threonine residues of various proteins, thereby initiating mucin-type O-linked glycosylation in the Golgi apparatus. GalNAc-T10 (Polypeptide N-acetylgalactosaminyltransferase 10), also known as UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10, is a 603 amino acid single-pass type II membrane protein that prefers Muc5Ac and EA2 peptide substrates. The N-terminal domain is involved in substrate binding and manganese coordination, while the C-terminal domain is involved in UDP-Gal binding and catalytic reaction. GalNAc-T10 is widely expressed, with highest levels found in small intestine. There are four isoforms of GalNAc-T10 that are produced as a result of alternative splicing events.

Gene ID: 55568