Nemo-Like Kinase antibody (pThr298) (HRP)

Catalog No. ABIN1711848

This anti-Nemo-Like Kinase antibody is a Rabbit Polyclonal antibody detecting Nemo-Like Kinase in WB, ELISA, IHC (p) and IHC (fro). Suitable for Human and Mouse.

Quick Overview for Nemo-Like Kinase antibody (pThr298) (HRP) (ABIN1711848)

Target: Nemo-Like Kinase (NLK)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nemo-Like Kinase antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Nemo-Like Kinase Antibody (HRP)

Binding Specificity: pThr298

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow,Sheep,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Nemo-like kinase around the phosphorylation site of Thr298

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Nemo-Like Kinase

Target: Nemo-Like Kinase (NLK)

Alternative Name: Nemo-like kinase

Background:

Synonyms: Nemo-like kinase Thr298, Nemo-like kinase T298, p-Nemo-like kinase Thr298, p-Nemo-like kinase T298, DKFZp761G1211, FLJ21033, LAK1, Nemo like kinase, Nemo-like kinase, Nlk, NLK_HUMAN, Protein LAK1, Serine/threonine protein kinase NLK, Serine/threonine-protein kinase NLK.

Background: Nemo-like kinase (NLK) plays a role in cell fate determination and is required for differentiation of bone marrow stromal cells. It acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. It may suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP. (referenced from swissprot)

Pathways: Ubiquitin Proteasome Pathway