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GUCY1A2 antibody (AA 401-500) (HRP)

Reactivity: Human WB, IHC (p), ELISA, IHC (fro) Host: Rabbit Polyclonal HRP
Catalog No. ABIN1712342
  • Target See all GUCY1A2 products
    GUCY1A2 (Guanylyl Cyclase alpha 2)
    Binding Specificity
    • 14
    • 4
    • 2
    • 1
    • 1
    AA 401-500
    Reactivity
    • 19
    • 3
    • 2
    Human
    Host
    • 19
    Rabbit
    Clonality
    • 19
    Polyclonal
    Conjugate
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GUCY1A2 antibody is conjugated to HRP
    Application
    • 18
    • 13
    • 13
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))
    Predicted Reactivity
    Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Rabbit
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human Guanylyl Cyclase alpha 2
    Isotype
    IgG
  • Application Notes
    WB 1:300-5000
    IHC-P 1:200-400
    IHC-F 1:100-500
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Handling Advice
    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    GUCY1A2 (Guanylyl Cyclase alpha 2)
    Abstract
    GUCY1A2 Products
    Synonyms
    GC-SA2 antibody, GUC1A2 antibody, guanylate cyclase 1 soluble subunit alpha 2 antibody, GUCY1A2 antibody
    Background

    Synonyms: GC SA2, GCS-alpha 2, GUC1A2, GUCSA2, GCYA2_HUMAN.

    Background: Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase. The soluble form, known as GCS or sGC, act as receptors for nitric oxide (NO). The membrane-bound receptor form, known as GC, are peptide hormone receptors. GCS is a cGMP-synthesizing enzyme, which is the major receptor for the neurotransmitter nitric oxide. It plays a crucial role in smooth muscle contractility, platelet reactivity and neurotransmission. GCS is a heme-containing heterodimer, consisting of one alpha subunit and one beta subunit. The heme moeity mediates NO activation, and this heme group also binds carbon monoxide (CO), which weakly stimulates the enzyme. Both NO and CO stimulation are enhanced by the allosteric activator 3-(5'-hydroxymethyl-2'furyl)-benzyl-indazole, YC-1. YC-1 can also stimulate GCS in a NO-independent manner. Both alpha and beta subunits are required for cGMP generation, and at least two isoforms exist for each subunit. Heterodimers consisting of alpha-1/beta-1 and alpha-2/beta-1 have been identified, and both display similar enzymatic activity. The distribution of the beta-2 subunit seems to be much more restricted than the beta-1 subunit, with predominant expression in kidney and liver.

    Gene ID
    2977
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