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ENOS antibody (pSer615) (HRP)

The Rabbit Polyclonal anti-ENOS antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect ENOS in samples from Human.
Catalog No. ABIN1712423

Quick Overview for ENOS antibody (pSer615) (HRP) (ABIN1712423)

Target

See all ENOS (NOS3) Antibodies
ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This ENOS antibody is conjugated to HRP

Application

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Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Binding Specificity

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    pSer615

    Cross-Reactivity

    Human

    Predicted Reactivity

    Dog,Rabbit

    Purification

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Ser615

    Isotype

    IgG
  • Application Notes

    WB 1:300-5000
    IHC-P 1:200-400
    IHC-F 1:100-500

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Buffer

    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Handling Advice

    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

    Alternative Name

    eNOS

    Background

    Synonyms: eNOS phospho S615, p-eNOS phospho S615, eNOS Ser615, cNOS, eNOS, Constitutive NOS, EC NOS, ecNOS, Endothelial nitric oxidase synthase, Endothelial nitric oxide synthase, Endothelial nitric oxide synthase 3, Endothelial NOS, Nitric oxide synthase 3 endothelial cell, Nitric oxide synthase 3, Nitric oxide synthase 3 endothelial cell, Nitric oxide synthase endothelial, nitric oxide synthase, endothelial, NOS 3, NOS III, NOS type III, NOS3, NOSIII, NOS3_HUMAN.

    Background: eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.

    Gene ID

    4846

    Pathways

    ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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