GLI4 antibody (AA 271-376) (HRP)

Catalog No. ABIN1712537

The Rabbit Polyclonal anti-GLI4 antibody has been validated for WB, ELISA, IHC (p), IHC (fro). It is suitable to detect GLI4 in samples from Human.

Quick Overview for GLI4 antibody (AA 271-376) (HRP) (ABIN1712537)

Target: GLI4 (GLI Family Zinc Finger Protein 4 (GLI4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI4 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GLI4 Antibody (HRP)

Binding Specificity: AA 271-376

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GLI4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GLI4

Target: GLI4 (GLI Family Zinc Finger Protein 4 (GLI4))

Alternative Name: GLI4

Background:

Synonyms: GLI 4, GLI Kruppel family member GLI 4, GLI Kruppel family member GLI4, GLI4, GLI4_HUMAN, HKR 4, HKR4, HKR4 protein, Krueppel related zinc finger protein 4, Krueppel-related zinc finger protein 4, Neural specic DNA binding protein, Oncogene HKR 4, Oncogene HKR4, Protein HKR 4, Protein HKR4, Zinc finger protein GLI 4, Zinc finger protein GLI4.

Background: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a krueppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. GLI-4, also known as HKR4, is a 376 amino acid protein that localizes to the nucleus and contains seven C2H2-type zinc fingers. Belonging to the krueppel C2H2-type zinc-finger protein family, GLI-4 may function as a transcriptional regulator, effectively activating or repressing the transcription of target genes. The gene encoding GLI-4 maps to human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies.

Gene ID: 2738