GORASP2 antibody (AA 151-250) (HRP)

Catalog No. ABIN1712546

The Rabbit Polyclonal anti-GORASP2 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect GORASP2 in samples from Mouse and Xenopus laevis.

Quick Overview for GORASP2 antibody (AA 151-250) (HRP) (ABIN1712546)

Target: GORASP2 (Golgi Reassembly Stacking Protein 2, 55kDa (GORASP2))

Reactivity: Mouse, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GORASP2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GORASP2 Antibody (HRP)

Binding Specificity: AA 151-250

Cross-Reactivity: Mouse, Xenopus laevis

Predicted Reactivity: Human,Rat,Cow,Sheep,Pig,Horse,Rabbit,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Golgi phosphoprotein 6

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GORASP2

Target: GORASP2 (Golgi Reassembly Stacking Protein 2, 55kDa (GORASP2))

Alternative Name: Golgi phosphoprotein 6

Background:

Synonyms: Golgi phosphoprotein 6, Golgi reassembly stacking protein 2, Golgi reassembly stacking protein 2, 55 kDa, Golgi reassembly stacking protein of 55 kDa, Golgi reassembly-stacking protein 2, Golgi reassembly-stacking protein of 55 kDa, GOLPH2, GOLPH6, GORASP2, GORS2_HUMAN, GRASP55, GRS2, p59, RP23-428O15.1.

Background: The Golgi apparatus is a highly complex organelle comprised of a stack of cisternal membranes on the secretory pathway from the ER to the cell surface. The structure is maintained by an exoskeleton or Golgi matrix constructed from a family of coiled-coil protein, the golgins and other peripheral membrane components such as GRASP55 and GRASP65 (1). GRASP55 (Golgi reassembly stacking protien or p59) is a component of the Golgi stacking machinery. GRASP55 is highly homologous to GRASP65 and contains two PDZ domains. GRASP55 is myristoylated and palmitoylated. Unlike GRASP65, GRASP55 does not have detectable binding with the vesicle docking protein GM130 and is located on the medial-Golgi rather than cis-Golgi. Both GRASP55 and GRASP65 function in the stacking of Golgi Cisternae (2,3). The novel coiled-coil protein golgin 45 interacts with GRASP55 and the GTP form of Rab 2, suggesting that GRASP55 and golgin 45 form a Rab 2 effector complex on medial-Golgi essential for normal protein transport and Golgi structure (4). ERK2 directly phosphorylates GRASP55, which is phosphorylated in mitotic cells, suggesting that mitogen-activated protein kinase kinase (MKK)/ERK pathway phosphorylates the Golgi during mitosis (5).

Gene ID: 26003