TRKA antibody (AA 101-200) (HRP)

Catalog No. ABIN1712572

Product Details anti-TRKA Antibody (HRP)

Target: TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))

Binding Specificity: AA 101-200

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRKA antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TrkA

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TRKA

Target: TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))

Alternative Name: TrkA (NTRK1 Products)

Background:

Synonyms: CIPA, CIPA, DKFZp781I14186, gp140trk, High affinity nerve growth factor receptor, High affinity nerve growth factor receptor precursor, High affinity nerve growth factor receptor precursor, MTC, MTC, neurotrophic tyrosine kinase receptor type 1, neurotrophic tyrosine kinase receptor type 1, Ntrk 1, Ntrk1, Ntrk1, NTRK1_HUMAN, Oncogene TRK, Oncogene TRK, p140 TrkA, p140-TrkA, TRK 1, Trk A, TRK, TRK, Trk-A, TRK1, TRK1 transforming tyrosine kinase protein, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor A, Tyrosine kinase receptor, Tyrosine Receptor Kinase A.

Background: The Trk family of nerve growth factor receptors includes Trk A(also referfed to as Trk A gp140),Trk B and Trk C. The prototype member of this gene family, Trk A, encodes a 140 kDa cell surface receptor , gp140, the expression of which is restricted in vivo to neurons of the sensory spinal and cranial gangliaof neurocrest origin. Nerve growth factor (NGF) stimulates tyrosine phosphorylation of Trk gp 140 in neural cell lines and in embryonic dorsal root ganglia. By comparison, BDNF and to a lesser extent, NT-3, but not NGF, can induce tyrosine phophorylayion of Trk B gp 145. The third member of the Trk receptor family, Trk C incodes a 140 kDa protein, Trk C gp140, that is preferentially expressed in brain tissue and primarily functions as a receptor for NT-3.An additional component of the Trk receptor complex, NGFR p175, binds to neurotrophic factors with low affinity but is required for efficient signaling. NGFR p175 accelerates Trk activation and may recruit downstream dffector molecules to the ligand-bound receptor complex.

Gene ID: 4914

Pathways: RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process