CD3 epsilon antibody (AA 11-100) (HRP)

Catalog No. ABIN1712649

This anti-CD3 epsilon antibody is a Rabbit Polyclonal antibody detecting CD3 epsilon in ELISA, IHC (fro) and IHC (p). Suitable for Human, Mouse and Pig.

Quick Overview for CD3 epsilon antibody (AA 11-100) (HRP) (ABIN1712649)

Target: CD3 epsilon (CD3E)

Reactivity: Human, Mouse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3 epsilon antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD3 epsilon Antibody (HRP)

Binding Specificity: AA 11-100

Cross-Reactivity: Human, Mouse, Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from pig CD3E

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background:

Synonyms: CD3, T-cell surface glycoprotein CD3 epsilon chain, CD3e

Background: The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008].

Gene ID: 397455

UniProt: Q7YRN2

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway