CD35 antibody (AA 1301-1400)

Catalog No. ABIN1713473

The Rabbit Polyclonal anti-CD35 antibody has been validated for ELISA, IHC (p), IHC (fro), ICC, IF (cc) and IF (p). It is suitable to detect CD35 in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for CD35 antibody (AA 1301-1400) (ABIN1713473)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD35 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CD35 Antibody

Binding Specificity: AA 1301-1400

Specificity: May recognize Complement component receptor 1-like protein in certain tissues, which is expressed in fetal liver and to a lesser extent in fetal spleen and thymus.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CR1/CD35

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD35 antibody (ABIN1713473)

Rovituso, Scheffler, Wunsch, Kleinschnitz, Dörck, Ulzheimer, Bayas, Steinman, Ergün, Kuerten: "CEACAM1 mediates B cell aggregation in central nervous system autoimmunity." in: Scientific reports, Vol. 6, pp. 29847, (2016) (PubMed).

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CD35

Background:

Synonyms: KN, C3BR, C4BR, CD35, Complement receptor type 1, C3b/C4b receptor, CR1

Background: Mediates cellular binding of particles and immune complexes that have activated complement.

Gene ID: 1378

UniProt: P17927

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity