Phenylalanyl-tRNA Synthetase, alpha Subunit (FARSA) (AA 51-150) antibody

Catalog No. ABIN1713494

The Rabbit Polyclonal anti- antibody has been validated for ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro). It is suitable to detect in samples from Human.

Quick Overview for Phenylalanyl-tRNA Synthetase, alpha Subunit (FARSA) (AA 51-150) antibody (ABIN1713494)

Target: Phenylalanyl-tRNA Synthetase, alpha Subunit (FARSA)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details

Binding Specificity: AA 51-150

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FARSLA/CML33

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Phenylalanyl-tRNA Synthetase, alpha Subunit (FARSA)

Alternative Name: FARSLA/CML33

Background:

Synonyms: CML 33, CML33, FARS, FARSA, FARSL, FRSA, PheHA, Phenylalanine tRNA ligase 1 alpha cytoplasmic, Phenylalanine tRNA ligase alpha chain, Phenylalanine tRNA synthetase alpha subunit, Phenylalanine tRNA synthetase like alpha subunit, Phenylalanyl tRNA synthetase alpha chain, Phenylalanyl tRNA synthetase alpha subunit, Phenylalanyl tRNA synthetase like alpha subunit, PheRS.

Background: Aminoacyl-tRNA synthetases consist of a family of enzymes that catalyze the specific aminoacylation of tRNA by their cognate amino acid in the initial step of ribosome-dependent protein biosynthesis. FARSLA, also known as FRSA, CML33, FARSL or PheHA (phenylalanyl-tRNA synthetase, alpha subunit), is a member of the class-II aminoacyl-tRNA synthetase family and is highly expressed in proliferating cells of bone marrow. FARSLA is a cytoplasmic phenylalanine-tRNA synthetase that functions as a heterodimer consisting of a catalytic alpha-subunit and a regulatory beta-subunit. The alpha-subunit is responsible for forming the amino acid binding pocket, mediating the ATP/aminoacyl adenylate binding, and interacts with the acceptor stem of the tRNA. FARSLA functions in a cell cycle-dependent and differentiation-dependent manner.

Gene ID: 10160