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FFAR2 antibody (AA 41-140)

The Rabbit Polyclonal anti-FFAR2 antibody has been validated for WB, ELISA, IHC (p), IF (p), FACS and IHC (fro). It is suitable to detect FFAR2 in samples from Mouse and Rat. There is 1 publication available.
Catalog No. ABIN1713521

Quick Overview for FFAR2 antibody (AA 41-140) (ABIN1713521)

Target

See all FFAR2 Antibodies
FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

Reactivity

  • 59
  • 20
  • 17
  • 3
  • 2
  • 1
  • 1
Mouse, Rat

Host

  • 78
  • 1
Rabbit

Clonality

  • 78
  • 1
Polyclonal

Conjugate

  • 32
  • 7
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This FFAR2 antibody is un-conjugated

Application

  • 54
  • 27
  • 25
  • 19
  • 15
  • 14
  • 13
  • 8
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  • 5
  • 5
  • 4
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Binding Specificity

    • 15
    • 15
    • 12
    • 8
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 41-140

    Cross-Reactivity

    Mouse, Rat

    Predicted Reactivity

    Human

    Purification

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic peptide derived from human GPR43

    Isotype

    IgG
  • Application Notes

    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Buffer

    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

    Expiry Date

    12 months
  • Kobayashi, Mikami, Kimura, Kamiyama, Morikawa, Yokoi, Kasuno, Takahashi, Taniguchi, Iwano: "Short-chain fatty acids, GPR41 and GPR43 ligands, inhibit TNF-α-induced MCP-1 expression by modulating p38 and JNK signaling pathways in human renal cortical epithelial cells." in: Biochemical and biophysical research communications, Vol. 486, Issue 2, pp. 499-505, (2017) (PubMed).

  • Target

    FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

    Alternative Name

    GPR43

    Background

    Synonyms: FFA2R, GPR43, Free fatty acid receptor 2, G-protein coupled receptor 43, FFAR2, FFA2, GPCR43

    Background: G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, the short chain fatty acids acetate, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins but also to the Gq family (PubMed:12496283, PubMed:12711604, PubMed:23589301). Its activation results in the formation of inositol 1,4,5-trisphosphate, the mobilization of intracellular calcium, the phosphorylation of the MAPK3/ERK1 and MAPK1/ERK2 kinases and the inhibition of intracellular cAMP accumulation. May play a role in glucose homeostasis by regulating the secretion of GLP-1, in response to short-chain fatty acids accumulating in the intestine. May also regulate the production of LEP/Leptin, a hormone acting on the central nervous system to inhibit food intake. Finally, may also regulate whole-body energy homeostasis through adipogenesis regulating both differentiation and lipid storage of adipocytes. In parallel to its role in energy homeostasis, may also mediate the activation of the inflammatory and immune responses by SCFA in the intestine, regulating the rapid production of chemokines and cytokines. May also play a role in the resolution of the inflammatory response and control chemotaxis in neutrophils. In addition to SCFAs, may also be activated by the extracellular lectin FCN1 in a process leading to activation of monocytes and inducing the secretion of interleukin-8/IL-8 in response to the presence of microbes (PubMed:21037097).

    Gene ID

    2867

    UniProt

    O15552
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