RBMY1F antibody (AA 54-160)

Catalog No. ABIN1713569

The Rabbit Polyclonal anti-RBMY1F antibody is suitable to detect RBMY1F in samples from Rat. It has been validated for ELISA, IF (cc), IF (p), IHC (fro), IHC (p) and ICC.

Quick Overview for RBMY1F antibody (AA 54-160) (ABIN1713569)

Target: RBMY1F (RNA Binding Motif Protein, Y-Linked, Family 1, Member F (RBMY1F))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBMY1F antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-RBMY1F Antibody

Binding Specificity: AA 54-160

Cross-Reactivity: Rat

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RBMY1F

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RBMY1F

Target: RBMY1F (RNA Binding Motif Protein, Y-Linked, Family 1, Member F (RBMY1F))

Alternative Name: RBMY1F

Background:

Synonyms: YRRM2, RNA binding mot protein, Y chromosome, family 1 member F/J, RNA binding mot protein, Y linked, family 1, member F, Y chromosome RNA recognition mot 2, YRRM2, RBY1F_HUMAN.

Background: The RBM (RNA-binding motif) gene family encodes proteins with an RNA binding motif. RBMY (RBM, Y chromosome) encodes a germ-cell specific nuclear protein involved in spermatogenesis. The RBM gene family, including RBMY1A, RBMY1B, RBMY1D, RBMY1E, RBMY1F, RBMY1H and RBMY1J, is comprised of at least 30 genes and pseudogenes, found on both arms of the Y chromosome. RBM X, an ancestral X chromosome homolog of the RBMY gene, encodes hnRNP G, which is widely expressed, whereas the RBMY gene evolved a male-specific function in spermatogenesis. Micro-deletions of the AZFb region of the Y chromosome, which contains a number of RBMY genes, usually result in severe consequences for spermatogenesis. RBM expression is localized to the nuclei of germ cells and RBM interacts with Tra2beta. Tra2beta is a ubiquitous activator of pre-mRNA splicing, but is most highly expressed in testis, suggesting a role for RBM in Tra2beta-dependent splicing in spermatocytes. The human RBMX gene maps to chromosome Xq26 and the RBMY gene family is found on all mammalian Y chomosomes.

Gene ID: 159163