Factor XII Light Chain (Light Chain) antibody

Catalog No. ABIN1713750

The Rabbit Polyclonal anti-Factor XII Light Chain antibody (ABIN1713750) specifically detects Factor XII Light Chain in WB, IF (p) and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Factor XII Light Chain (Light Chain) antibody (ABIN1713750)

Target: Factor XII Light Chain

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: Light Chain

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Coagulation factor XIIa light chain

Isotype: IgG

Application Details

Application Notes: WB: 1:100-1000, IHC-P: 1:100-500, IF(IHC-P): 1:50-200
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details

Target: Factor XII Light Chain

Background:

Synonyms: Factor XII, Coagulation factor XIIa light chain, Factor XII, Coagulation factor XII, Factor XII light chain, F12, F12 deficiency, FA12_HUMAN, Factor XII deficiency, HAE3, HAEX, HAF, HAF deficiency, Hageman factor, Beta-factor XIIa part 2.

Background: This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008].

Gene ID: 2161