CPA3 antibody (AA 101-200)

Catalog No. ABIN1713752

The Rabbit Polyclonal anti-CPA3 antibody has been validated for WB, ELISA, ICC, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect CPA3 in samples from Rat. There is 1 publication available.

Quick Overview for CPA3 antibody (AA 101-200) (ABIN1713752)

Target: CPA3 (Carboxypeptidase A3 (Mast Cell) (CPA3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPA3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CPA3 Antibody

Binding Specificity: AA 101-200

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CPA3/MC-CPA

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CPA3 antibody (ABIN1713752)

Mancini, Gohlke, Garcia-Carrizo, Zagoriy, Stephanowitz, Schulz: "Identification of biomarkers of brown adipose tissue aging highlights the role of dysfunctional energy and nucleotide metabolism pathways." in: Scientific reports, Vol. 11, Issue 1, pp. 19928, (2021) (PubMed).

Target Details for CPA3

Target: CPA3 (Carboxypeptidase A3 (Mast Cell) (CPA3))

Alternative Name: CPA3/MC-CPA

Background:

Synonyms: carboxypeptidase A, Carboxypeptidase A3, carboxypeptidase A3 mast cell, CBPA3_HUMAN, Cpa3, Mast cell carboxypeptidase A, mast cell carboxypeptidase A3, MC CPA, MC-CPA.

Background: Carboxypeptidase A (CPA) is a pancreatic exopeptidase which hydrolyses the peptide bond adjacent to the C-terminal end in polypeptide chains. Mast cell carboxypeptidase A (MC-CPA), a part of the peptidase M14 family, is a highly conserved metalloprotease localized to the secretory granules, along with trytases and chymases. MC-CPA is stored as an active enzyme in the granule and is released, along with other inflammatory mediators, upon mast cell degranulation. MC-CPA mirrors pancreatic carboxypeptidase A in cleaving COOH-terminal aromatic and aliphatic amino acid residues. The optimum pH of MC-CPA is between neutral and basic, depending upon the substrate. The MC-CPA gene, CPA3, resides on chromosome 3 and contains 11 exons.

Gene ID: 1359

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones