DNMT3L antibody (AA 131-230)

Catalog No. ABIN1713766

This Rabbit Polyclonal antibody specifically detects DNMT3L in WB, ELISA, IF (cc), IF (p), ICC, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for DNMT3L antibody (AA 131-230) (ABIN1713766)

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DNMT3L Antibody

Binding Specificity: AA 131-230

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Dnmt3L

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DNMT3L

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Alternative Name: Dnmt3L

Background:

Synonyms: Cytosine 5 methyltransferase 3 like protein, DNA cytosine 5 methyltransferase 3 like, DNA cytosine-5-methyltransferase 3-like, DNA Cytosine 5 Methyltransferase 3 Like Protein, DNA Methyltransferase 3 Like Protein, DNM3L_HUMAN, Dnmt 3L, Dnmt3l, Human cytosine 5 methyltransferase 3 like protein, MGC1090.

Background: Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines, thereby repressing expression of the target gene. Dnmt3L (DNA (cytosine-5)-methyltransferase 3-like) is a 387 amino acid protein that contains one ADD-type zinc finger and is a member of the Dnmt family. Localized to the nucleus and expressed at lows levels in thymus, testis and ovary, Dnmt3L does not exhibit DNA methyltransferase activity, but is able to stimulate de novo methylation by Dnmt3 and is thought to play a key role in the establishment of genomic imprints. Additionally, Dnmt3L interacts with histone deacetylase 1 (HDAC1) and, through this interaction, mediates transcriptional repression. Multiple isoforms of Dnmt3L exist due to alternative splicing events.

Gene ID: 29947