GAMT antibody (AA 101-200)

Catalog No. ABIN1713803

The Rabbit Polyclonal anti-GAMT antibody has been validated for WB, ELISA, ICC, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect GAMT in samples from Human.

Quick Overview for GAMT antibody (AA 101-200) (ABIN1713803)

Target: GAMT (Guanidinoacetate N-Methyltransferase (GAMT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAMT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GAMT Antibody

Binding Specificity: AA 101-200

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GAMT

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for GAMT

Target: GAMT (Guanidinoacetate N-Methyltransferase (GAMT))

Alternative Name: GAMT

Background:

Synonyms: PIG2, CCDS2, TP53I2, HEL-S-20, Guanidinoacetate N-methyltransferase, GAMT

Background: In the creatine biosynthesis pathway, glycine is converted to guanidinoacetate by amidinotransferase, and guanidinoacetate is then converted to creatine by Guanidinoacetate N-methyltransferase (GAMT). GAMT, a methyltransferase, uses S-adenosylmethionine as the methyl donor for this reaction. Methyltransferases are a type of transferase enzyme which transfers a methyl group to nucleic bases in DNA or amino acids in protein. Encoding a 236 amino acid protein, the human GAMT gene maps to chromosome 19p13.3. Defects in the GAMT gene leads to GAMT deficiency, which is associated with guanidinoacetate accumulation and decreased levels of creatine excretion in brain. Such biochemical changes are thought to lead to various neurological syndromes and muscular hypotonia.

Gene ID: 2593

UniProt: Q14353