PIK3AP1 antibody (AA 601-700)

Catalog No. ABIN1713881

The Rabbit Polyclonal anti-PIK3AP1 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p), ICC and IHC (fro). It is suitable to detect PIK3AP1 in samples from Human.

Quick Overview for PIK3AP1 antibody (AA 601-700) (ABIN1713881)

Target: PIK3AP1 (phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3AP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PIK3AP1 Antibody

Binding Specificity: AA 601-700

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PIK3AP1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PIK3AP1

Target: PIK3AP1 (phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1))

Alternative Name: PIK3AP1

Background:

Synonyms: B cell adaptor protein, B-cell adapter for phosphoinositide 3-kinase, B-cell phosphoinositide 3-kinase adapter protein 1, BCAP, BCAP_HUMAN, OTTHUMP00000020175, OTTHUMP00000020176, Phosphoinositide 3 kinase adaptor protein 1, Phosphoinositide 3-kinase adapter protein 1, PIK3AP1, RP11-34E5.3.

Background: B cell adaptor for phosphoinositide 3-kinase (BCAP) is a tyrosine kinase substrate that bridges B cell receptor (BCR) associated kinases to the PIK3 pathway. Syk, Btk or Lyn-dependent tyrosine phosphorylation of BCAP, provides binding sites for the p85 subunit of PIK3. BCAP mRNA is present in mouse spleen, thymus, liver, lung, macrophage and B cell lines. Human BCAP maps to chromosome 10q24.2.

Pathways: Activation of Innate immune Response, BCR Signaling, Warburg Effect