alpha Adducin antibody (pThr445)

Catalog No. ABIN1713941

The Rabbit Polyclonal anti-alpha Adducin antibody has been validated for ELISA, IHC (p), IF (cc), IF (p), ICC and IHC (fro). It is suitable to detect alpha Adducin in samples from Human.

Quick Overview for alpha Adducin antibody (pThr445) (ABIN1713941)

Target: alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Adducin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-alpha Adducin Antibody

Binding Specificity: pThr445

Predicted Reactivity: Human,Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human alpha Adducin around the phosphorylation site of Thr445

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for alpha Adducin

Target: alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

Alternative Name: alpha Adducin

Background:

Synonyms: Adducin phospho T445, p-Adducin phospho T445, ADD1, ADDA, Adducin 1 alpha, Adducin 1, Alpha adducin, ADDA_HUMAN.

Background: Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms, however, not all variants have been fully described.

Gene ID: 118

UniProt: P35611

Pathways: Negative Regulation of Hormone Secretion, Regulation of Actin Filament Polymerization, Regulation of Lipid Metabolism by PPARalpha, ER-Nucleus Signaling