FBXO45 antibody (AA 110-210)

Catalog No. ABIN1714025

The Rabbit Polyclonal anti-FBXO45 antibody has been validated for ELISA, WB, ICC, IF (cc), IF (p), IHC (fro) and IHC (p). It is suitable to detect FBXO45 in samples from Mouse and Rat.

Quick Overview for FBXO45 antibody (AA 110-210) (ABIN1714025)

Target: FBXO45 (F-Box Protein 45 (FBXO45))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO45 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FBXO45 Antibody

Binding Specificity: AA 110-210

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Sheep,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FBXO45

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for FBXO45

Target: FBXO45 (F-Box Protein 45 (FBXO45))

Alternative Name: FBXO45

Background:

Synonyms: F box protein 45, F-box only protein 45, F-box/SPRY domain-containing protein 1, FBSP1_HUMAN, FBX45, fbxo45, hFbxo45.

Background: Belonging to the F-box family of proteins, FBXO45 is a 286 amino acid protein that contains one C-terminal F-box domain. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein)-type E3 ubiquitin ligase complex and are involved in substrate recognition and protein recruitment for ubiquitination. They are members of a larger family of proteins that are involved in the regulation of a wide variety of cellular mechanisms, including the cell cycle, the immune response, signaling cascades and developmental processes. They function by targeting proteins, such as cyclins, cyclin-dependent kinase inhibitors, IB- and -catenin, for degradation by the proteasome after ubiquitination. Via its F-box domain, FBXO45 can directly interact with Skp1 p19 and CUL-1. FBXO45 has been shown to be an estrogen-induced gene.

Gene ID: 200933

UniProt: P0C2W1