PBX1 antibody (AA 221-340)

Catalog No. ABIN1714066

This Rabbit Polyclonal antibody specifically detects PBX1 in WB, ELISA, IHC (p), ICC, IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for PBX1 antibody (AA 221-340) (ABIN1714066)

Target: PBX1 (Pre-B-Cell Leukemia Homeobox Protein 1 (PBX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PBX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PBX1 Antibody

Binding Specificity: AA 221-340

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Horse,Chicken,Rabbit,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PBX1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PBX1

Target: PBX1 (Pre-B-Cell Leukemia Homeobox Protein 1 (PBX1))

Alternative Name: PBX1

Background:

Synonyms: DKFZp686B09108, Homeo box protein PBX1, Homeo box protein PRL, Homeobox protein PBX 1, Homeobox protein PBX1, Homeobox protein PRL, MGC126627, PBX 1, Pbx1, PBX1_HUMAN, Pre B cell leukemia homeobox 1, Pre B cell leukemia transcription factor 1, Pre-B-cell leukemia transcription factor 1, PRL.

Background: Pbx 1, 2, 3 and 4 are members of the TALE (three amino acid loop extension) family of homeodomain-containing proteins. Human pre-B cell acute leukemias are frequently associated with a t(1,19)(q23,p13.3) chromosomal rearrangement, which creates a chimeric gene encoding a fusion between the E2A and Pbx 1 gene products. Pbx 2 and Pbx 3 share 92 % and 94 % respective identities with Pbx 1 over a 266 amino acid region flanking their homeobox domains, while all three proteins are quite divergent at their amino- and carboxy-termini. Two forms of Pbx 1 and Pbx 3 each differ primarily in their carboxy-termini and result from alternative mRNA splicing. Unlike other homeotic selector genes which are expressed transiently during development and differentiation, Pbx gene transcripts are ubiquitously expressed in both fetal and adult tissues and cell lines. Additionally, Pbx 2 and Pbx 3 transcripts are detected in lymphoid cells, which do not express Pbx 1. Pbx 4 expression is confined to the testis, especially to spermatocytes in the pachytene stage of the first meiotic prophase.

Gene ID: 5087