ASAH1 antibody (AA 301-395)

Catalog No. ABIN1714104

This anti-ASAH1 antibody is a Rabbit Polyclonal antibody detecting ASAH1 in WB, ELISA, IHC (p), IF (cc), IF (p), ICC and IHC (fro). Suitable for Mouse.

Quick Overview for ASAH1 antibody (AA 301-395) (ABIN1714104)

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASAH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ASAH1 Antibody

Binding Specificity: AA 301-395

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Acid ceramidase subunit beta

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ASAH1

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Alternative Name: ASAH1

Background:

Synonyms: AC, ACDase, Acid CDase, Acid ceramidase, Acid ceramidase precursor, Acid ceramidase subunit beta, Acylsphingosine deacylase, ASAH 1, ASAH, ASAH1, ASAH1_HUMAN, FLJ21558, FLJ22079, N acylsphingosine amidohydrolase acid ceramidase 1, N acylsphingosine amidohydrolase 1, N acylsphingosine amidohydrolase, N-acylsphingosine amidohydrolase, PHP, PHP32, Putative 32 kDa heart protein.

Background: Acid ceramidase catalyzes the degradation of ceramide in normal tissues, and deficiency leads to accumulation of ceramide in tissues, a hallmark of Farber disease. Effected individuals experience early onset joint problems and neurological problems, owing to mutations in the acid ceramidase gene. Bioinformatic analysis of gene expression also reveals acid ceramidase to be among the 5 most important genes associated with melanoma. In addition to ceramide hydrolysis, purified acid ceramidase also exhibits the ability to catalyze ceramide synthesis, utilizing [14C]lauric acid and sphingosine as substrates. Interestingly, pH regulates which reaction is favored, for hydrolysis the pH optimum is 4.5, whereas for the reverse reaction favors a pH of 5.5, further supporting a complex and central role for acid ceramidase in sphingolipid metabolism.

Gene ID: 427