Galnt1 antibody (AA 101-200)

Catalog No. ABIN1714179

The Rabbit Polyclonal anti-Galnt1 antibody (ABIN1714179) specifically detects Galnt1 in WB, IF (cc), IF (p), ELISA, IHC (fro), IHC (p) and ICC. The antibody is reactive with Human samples.

Quick Overview for Galnt1 antibody (AA 101-200) (ABIN1714179)

Target: Galnt1 (Udp-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 1 (GalNAc-T1) (Galnt1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Galnt1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-Galnt1 Antibody

Binding Specificity: AA 101-200

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GALNT1/GalNAc-T1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Galnt1

Target: Galnt1 (Udp-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 1 (GalNAc-T1) (Galnt1))

Alternative Name: GALNT1/GalNAc-T1

Background:

Synonyms: GALNAC T1, GalNAc transferase 1, GalNAc-T1, GALNT 1, GALNT1, GALT1_HUMAN, Polypeptide GalNAc transferase 1, Polypeptide N acetylgalactosaminyltransferase 1, Polypeptide N-acetylgalactosaminyltransferase 1 soluble form, pp GaNTase 1, pp-GaNTase 1, Protein UDP acetylgalactosaminyltransferase 1, Protein-UDP acetylgalactosaminyltransferase 1, UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 1, UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 GalNAc T1, UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1.

Background: The UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes are substrate-specific proteins that catalyze the transfer of GalNAc (N-acetylgalactosamine) to serine and threonine residues onto various proteins, thereby initiating mucin-type O-linked glycosylation in the Golgi apparatus. GalNAc-T1, also known as GALNT1 (Polypeptide N-acetylgalactosaminyltransferase 1), is a ubiquitously expressed 559 amino acid single-pass type II membrane protein that localizes to the Golgi apparatus and, like other GalNAc-Ts, contains a stem region and a C-terminal ricin/lectin-like domain. GalNAc-T1 catalyzes the first reaction in O-linked oligosaccharide biosynthesis, namely the transfer of an N-acetyl-D-galactosamine residue to a protein acceptor. GalNAc-T1 uses calcium and manganese as cofactors. Due to alternative splicing events, two GalNAc-T1 isoforms are expressed.

Gene ID: 2589