Galnt1 antibody (AA 101-200)

Catalog No. ABIN1714179

This anti-Galnt1 antibody is a Rabbit Polyclonal antibody detecting Galnt1 in WB, IF (cc), IF (p), ELISA, IHC (fro), IHC (p) and ICC. Suitable for Human.

Quick Overview for Galnt1 antibody (AA 101-200) (ABIN1714179)

Target: Galnt1 (Udp-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 1 (GalNAc-T1) (Galnt1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Galnt1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-Galnt1 Antibody

Binding Specificity: AA 101-200

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GALNT1/GalNAc-T1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Galnt1

Target: Galnt1 (Udp-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 1 (GalNAc-T1) (Galnt1))

Alternative Name: GALNT1/GalNAc-T1

Background:

Synonyms: GALNAC T1, GalNAc transferase 1, GalNAc-T1, GALNT 1, GALNT1, GALT1_HUMAN, Polypeptide GalNAc transferase 1, Polypeptide N acetylgalactosaminyltransferase 1, Polypeptide N-acetylgalactosaminyltransferase 1 soluble form, pp GaNTase 1, pp-GaNTase 1, Protein UDP acetylgalactosaminyltransferase 1, Protein-UDP acetylgalactosaminyltransferase 1, UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 1, UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 GalNAc T1, UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1.

Background: The UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes are substrate-specific proteins that catalyze the transfer of GalNAc (N-acetylgalactosamine) to serine and threonine residues onto various proteins, thereby initiating mucin-type O-linked glycosylation in the Golgi apparatus. GalNAc-T1, also known as GALNT1 (Polypeptide N-acetylgalactosaminyltransferase 1), is a ubiquitously expressed 559 amino acid single-pass type II membrane protein that localizes to the Golgi apparatus and, like other GalNAc-Ts, contains a stem region and a C-terminal ricin/lectin-like domain. GalNAc-T1 catalyzes the first reaction in O-linked oligosaccharide biosynthesis, namely the transfer of an N-acetyl-D-galactosamine residue to a protein acceptor. GalNAc-T1 uses calcium and manganese as cofactors. Due to alternative splicing events, two GalNAc-T1 isoforms are expressed.

Gene ID: 2589