NET1 antibody

Catalog No. ABIN1714215

This anti-NET1 antibody is a Rabbit Polyclonal antibody detecting NET1 in WB, IF (p) and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for NET1 antibody (ABIN1714215)

Target: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NET1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NET1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NET1

Isotype: IgG

Application Details

Application Notes: WB: (1:100-1000), IHC-P: (1:100-500), IF(IHC-P): (1:50-200)
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for NET1

Target: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Alternative Name: NET1

Background:

Synonyms: NAT1, NET, NET, NET1, Norepinephrine transporter, SLC6A2, SLC6A5, SLC6A5, Sodium dependent noradrenaline transporter, Solute carrier family 6 neurotransmitter transporter norepinephrine member 5, Solute carrier family 6 member 2, Solute carrier family 6 member 2.

Background: Catecholamine, a term used for the hormone adrenaline and its sequentially hydroxylated form noradrenaline, is involved in fight or flight responses. Noradrenaline is released from the post ganglionic sympathetic nerve endings and exerts its effects locally in the immediate vicinity of its release. In the CNS, noradrenaline is involved in a number of physiological responses including mood, sleep regulation, alertness and arousal, both cognitive and non-cognitive expression of behaviors, and control of the endocrine and autonomic nervous systems. Peripherally, noradrenaline is present in sympathetic nerve endings and has full control of the sympathetic nervous system. Noradrenaline released from pre-synaptic nerve terminals is reabsorbed (70-90 % ) by noradrenaline transporters and its biological effects are terminated. The noradrenaline transport via noradrenaline transporters is an active, Na+/Cl- dependent transport process mediated by noradrenaline transporters. Noradrenaline transporters constitute the primary mechanism for inactivation of synaptically released noradrenaline, are targets for multiple antidepressants and psychostimulants, and are deficient in affective and autonomic disorders. In rat brain, noradrenaline transporter is expressed in noradrenergic neuronal somata, axons and dendrites, and hippocampus and cortex, but is absent from epinephrine- and dopamine-containing neurons. At least 13 genetic variations have been reported in the noradrenaline transporter protein that affect noradrenaline re-uptake and concentrations in cerebrospinal fluid in humans. The association between these genetic variations in noradrenaline transporters and several psychiatric and cardiovascular disorders is just emerging. Recently, a single amino acid mutation (hNET-A457P) showed deficiency in noradrenaline transport in an orthostatic intolerance patient. Noradrenaline transporter protein consists of 617 amino acids and has 12 trans-membrane domains.

Gene ID: 6530

Pathways: Neurotrophin Signaling Pathway