CTLA4 antibody (AA 75-170)

Catalog No. ABIN1714514

The Rabbit Polyclonal anti-CTLA4 antibody has been validated for FACS, WB, ELISA, IHC (p) and IF (p). It is suitable to detect CTLA4 in samples from Human, Mouse and Rat. There are 2+ publications available.

Quick Overview for CTLA4 antibody (AA 75-170) (ABIN1714514)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CTLA4 Antibody

Binding Specificity: AA 75-170

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CTLA-4/CD152

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CTLA4 antibody (ABIN1714514)

Schlößer, Drebber, Kloth, Thelen, Rothschild, Haase, Garcia-Marquez, Wennhold, Berlth, Urbanski, Alakus, Schauss, Shimabukuro-Vornhagen, Theurich, Warnecke-Ebertz, Stippel, Zippelius, Büttner, Hallek et al.: "Immune checkpoints programmed death 1 ligand 1 and cytotoxic T lymphocyte associated molecule 4 in gastric adenocarcinoma. ..." in: Oncoimmunology, Vol. 5, Issue 5, pp. e1100789, (2016) (PubMed).

Tamaka, Seike, Hagiwara, Sato, Ohtsu: "Histamine suppresses regulatory T cells mediated by TGF-? in murine chronic allergic contact dermatitis." in: Experimental dermatology, Vol. 24, Issue 4, pp. 280-4, (2015) (PubMed).

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CTLA4

Background:

Synonyms: CD, GSE, GRD4, CD152, CTLA-4, IDDM12, CELIAC3, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA4

Background: Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.

Gene ID: 1493

UniProt: P16410

Pathways: Cancer Immune Checkpoints