ETF1 antibody (AA 341-437)

Catalog No. ABIN1714530

This anti-ETF1 antibody is a Rabbit Polyclonal antibody detecting ETF1 in ELISA, ICC, IHC (p), IF (cc), IF (p) and IHC (fro). Suitable for Xenopus laevis and Pufferfish.

Quick Overview for ETF1 antibody (AA 341-437) (ABIN1714530)

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Reactivity: Xenopus laevis, Pufferfish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ETF1 antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ETF1 Antibody

Binding Specificity: AA 341-437

Cross-Reactivity: Pufferfish, Xenopus laevis

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Horse,Zebrafish,Arabidopsis Thaliana

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human eRF1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ETF1

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Alternative Name: eRF1

Background:

Synonyms: Cl1 protein, D5S1995, ERF, eRF1, ERF1_HUMAN, ETF1, Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, Eukaryotic translation termination factor 1, MGC111066, Polypeptide chain release factor 1, Protein Cl1, RF1, Sup45 yeast omnipotent suppressor 45 homolog like 1, SUP45L1, TB3 1, TB3-1.

Background: Translation is carried out by the ribosome and several associated protein factors through three consecutive steps: initiation, elongation and termination. Termination of protein synthesis takes place when the ribosomal A site is occupied simultaneously by one of three stop codons and by a class 1 translation termination factor. In eukaryotes, this termination factor is the eukaryotic release factor 1 (eRF1), a protein that promotes hydrolysis of the last peptidyl-tRNA on the ribosome. eRF1 activity is stimulated by the association with the GTP-binding protein eRF3. eRF1 forms a quaternary complex with eRF3, GTP and the ribosome. This complex performs a dual role, where, in the GTP state,????????? it controls the positioning of eRF1 toward the stop codon and peptidyl-tRNA, and, in the GDP state,????????? it promotes the release of the eRFs from the ribosome. eRF1 contains a highly conserved Asn-Ile-Lys-Ser (NIKS) tetrapeptide, which is essential for the interaction of eRF1 with the ribosome. The gene encoding human eRF1 maps to chromosome 5q31.2.

Gene ID: 2107