GFI1 antibody (AA 301-400)

Catalog No. ABIN1714623

This anti-GFI1 antibody is a Rabbit Polyclonal antibody detecting GFI1 in WB, ELISA, IHC (p), ICC, IF (cc), IF (p) and IHC (fro). Suitable for Rat, Mouse and Xenopus laevis.

Quick Overview for GFI1 antibody (AA 301-400) (ABIN1714623)

Target: GFI1 (Growth Factor Independent 1 (GFI1))

Reactivity: Rat, Mouse, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GFI1 Antibody

Binding Specificity: AA 301-400

Cross-Reactivity: Mouse, Rat, Xenopus laevis

Predicted Reactivity: Human,Dog,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GFI1/ZNF163

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for GFI1

Target: GFI1 (Growth Factor Independent 1 (GFI1))

Alternative Name: GFI1/ZNF163

Background:

Synonyms: GFI 1, GFI1, GFI-1, GFI1_HUMAN, Growth factor independence 1, growth factor independent 1, Growth factor independent protein 1, SCN2, Zinc finger protein 163, Zinc finger protein Gfi 1, Zinc finger protein Gfi-1, Zinc finger protein Gfi1, ZNF 163, ZNF163.

Background: Growth factor independent 1 (Gfi-1) is a transcriptional repressor that specifically binds to the DNA consensus sequence TAAATCAC(A/T)GCA. The carboxy-terminus of Gfi-1 contains six C2H2-type zinc finger motifs, and zinc fingers 3, 4 and 5 are required for the binding of Gfi-1 to its DNA binding site. Gfi-1 also contains a 20 amino acid SNAG domain which mediates transcriptional repression. It represses Bax at the mRNA and protein levels, resulting in the inhibition of cell death. Gfi1 is expressed outside the lymphoid system in granulocytes and activated macrophages. Gfi-1B, a related protein, is a transcriptional repressor primarily expressed in bone marrow and spleen. Gfi-1B is a direct repressor of the p21 promoter and the Socs 1 and 3 promoters. The genes encoding human Gfi-1 and Gfi-1B map to chromosome 1p22 and 9q34.3, respectively.

Gene ID: 2672