SP6 antibody (AA 201-300)

Catalog No. ABIN1714877

The Rabbit Polyclonal anti-SP6 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) and ICC. It is suitable to detect SP6 in samples from Human.

Quick Overview for SP6 antibody (AA 201-300) (ABIN1714877)

Target: SP6 (Sp6 Transcription Factor (SP6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-SP6 Antibody

Binding Specificity: AA 201-300

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SP6

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SP6

Target: SP6 (Sp6 Transcription Factor (SP6))

Alternative Name: SP6

Background:

Synonyms: MGC119665, Epfn, EPIPROFIN, KLF14, Krueppel-like factor 14, MGC119662, MGC119663, MGC119664, Sp6, Sp6 transcription factor, SP6_HUMAN, Transcription factor Sp6.

Background: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. Sp6, also known as EPFN, EPIPROFIN or KLF14, is a 376 amino acid protein that localizes to the nucleus and contains three C2H2-type zinc fingers. Expressed ubiquitously with higher expression in developing teeth, hair follicles and limb buds, Sp6 functions to bind GC-rich sequences and related GT and CACCC boxes, thereby promoting cellular proliferation. Human Sp6 shares 96 % sequence homology with its mouse counterpart, suggesting a conserved role between species. The gene encoding Sp6 maps to human chromosome 17, which comprises over 2.5 % of the human genome and encodes over 1,200 genes.