UBE2Z antibody (AA 151-259)

Catalog No. ABIN1714891

This Rabbit Polyclonal antibody specifically detects UBE2Z in WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p), ICC. It exhibits reactivity toward Human.

Quick Overview for UBE2Z antibody (AA 151-259) (ABIN1714891)

Target: UBE2Z (Ubiquitin-Conjugating Enzyme E2Z (UBE2Z))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2Z antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-UBE2Z Antibody

Binding Specificity: AA 151-259

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human USE1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for UBE2Z

Target: UBE2Z (Ubiquitin-Conjugating Enzyme E2Z (UBE2Z))

Alternative Name: USE1

Background:

Synonyms: 2010315L10Rik, 5730403H22Rik, AV002165, D12, Ed2, Embryonic development factor 2, MDS032, P31, Protein D12, Protein p31, putative MAPK activating protein PM26, Putative MAPK-activating protein PM26, Q-snare, RGD1306660, SLT1, SNARE-like tail-anchored protein 1 homolog, Unconventional SNARE in the ER 1 homolog S. cerevisiae, Unconventional SNARE in the ER 1 homolog, Use1, USE1 like protein, Use1 unconventional SNARE in the ER 1 homolog S. cerevisiae, USE1-like protein, USE1_HUMAN, USE1L, Vesicle transport protein USE1.

Background: In eukaryotic cells, the Golgi apparatus receives newly synthesized proteins from the endoplasmic reticulum (ER) and, after covalent modification, delivers them to their destination in the cell. For membrane-directed proteins this process is believed to be carried out via vesicular transport. Correct vesicular transport is determined by specific pairing of vesicle-associated SNAREs (v-SNAREs) with those on the target membrane (t-SNAREs). Unconventional SNARE in the ER 1, also known as USE1 or protein p31, is a 259 amino acid t-SNARE that forms a larger complex with ZW10, RINT-1 and Syntaxin 18. Upon Mg2+-AP treatment in the presence of NSF and ?SNAP, ZW10, RINT-1 and USE1 dissociate from Syntaxin 18. USE1 is a single-pass type IV membrane protein that is localized to the endoplasmic reticulum membrane. Three named isoforms exist for USE1 as a result of alternative splicing events.

Gene ID: 55850