UBE2Z antibody (Middle Region)

Catalog No. ABIN2778279

This Rabbit Polyclonal antibody specifically detects UBE2Z in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig, Horse, Cow, Dog and Zebrafish (Danio rerio).

Quick Overview for UBE2Z antibody (Middle Region) (ABIN2778279)

Target: UBE2Z (Ubiquitin-Conjugating Enzyme E2Z (UBE2Z))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Cow, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2Z antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-UBE2Z Antibody

Binding Specificity: Middle Region

Purpose: USE1 Antibody - middle region

Sequence: ARSLKTNTLA AQSVIKKDNQ TLSHSLKMAD QNLEKLKTES ERLEQHTQKS

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against USE1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human USE1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP34134-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBE2Z

Target: UBE2Z (Ubiquitin-Conjugating Enzyme E2Z (UBE2Z))

Alternative Name: USE1

Background:

Background Information: MDS032 belongs to the USE1 family and is a component of a SNARE complex which may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.

Gene Name: Unconventional SNARE in the ER 1 homolog (S. cerevisiae)

Alternative Symbols: D12, P31, SLT1, MDS032

Protein Name: Vesicle transport protein USE1

Molecular Weight: 29kDa

Gene ID: 55850

NCBI Accession: NP_060937

UniProt: Q9NZ43