ESAT6 (AA 21-95) antibody

Catalog No. ABIN1715132

There is 1 publication for this product available. The Rabbit Polyclonal anti-ESAT6 antibody is suitable to detect ESAT6 in samples from Bacteria, Mouse and Mycobacteria. It has been validated for WB, ELISA, ICC, IF (cc), IF (p), IHC (fro) and IHC (p).

Quick Overview for ESAT6 (AA 21-95) antibody (ABIN1715132)

Target: ESAT6

Reactivity: Bacteria, Mouse, Mycobacteria

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 21-95

Cross-Reactivity: Bacteria, Mouse

Cross-Reactivity (Details): Mycobacterium

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Mycobacterium tuberculosis ESAT6

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

Koyama, Ito, Hasegawa, Eriguchi, Inaba, Ushigusa, Sugiura: "Exosomes derived from tumor cells genetically modified to express Mycobacterium tuberculosis antigen: a novel vaccine for cancer therapy." in: Biotechnology letters, Vol. 38, Issue 11, pp. 1857-1866, (2016) (PubMed).

Target Details

Target: ESAT6

Background:

Synonyms: 6 kDa early secretory antigenic target, 6 kDa early secretory antigenic target ESXA, ESAT 6, esaT6, ESXA, ESXA_MYCTU.

Background: Mycobacterium tuberculosis is a slow-growing obligate aerobic bacillus that causes most cases of tuberculosis (TB). It is a small, rod-like microbe that can withstand weak disinfectants and survive in a dry state for weeks, but can only grow within a host organism. M. tuberculosis has a thick waxy cell wall that is responsible for the typical caseous granuloma formation in tuberculosis. ESAT6 is a protein secreted by M. tuberculosis that induces a strong immune response in infected organisms, making it a strong candidate for the development of a vaccine against tuberculosis. ESAT6 may also be useful in the detection of the disease. Deletion of the ESAT6 gene leads to the loss of virulence of M. tuberculosis.