Chemerin antibody (AA 101-163)

Catalog No. ABIN1715135

There are 3+ publications for this product available. The Rabbit Polyclonal anti-Chemerin antibody is suitable to detect Chemerin in samples from Rat. It has been validated for WB, ELISA, IF (cc), IF (p), ICC, IHC (fro) and IHC (p).

Quick Overview for Chemerin antibody (AA 101-163) (ABIN1715135)

Target: Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Chemerin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Chemerin Antibody

Binding Specificity: AA 101-163

Cross-Reactivity: Rat

Predicted Reactivity: Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat chemerin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Chemerin antibody (ABIN1715135)

Gonzalvo-Feo, Del Prete, Pruenster, Salvi, Wang, Sironi, Bierschenk, Sperandio, Vecchi, Sozzani: "Endothelial cell-derived chemerin promotes dendritic cell transmigration." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 5, pp. 2366-73, (2014) (PubMed).

Akamata, Asano, Taniguchi, Yamashita, Saigusa, Nakamura, Noda, Aozasa, Toyama, Takahashi, Ichimura, Sumida, Tada, Sugaya, Kadono, Sato: "Increased expression of chemerin in endothelial cells due to Fli1 deficiency may contribute to the development of digital ulcers in systemic sclerosis." in: Rheumatology (Oxford, England), (2014) (PubMed).

Krautbauer, Wanninger, Eisinger, Hader, Beck, Kopp, Schmid, Weiss, Dorn, Buechler: "Chemerin is highly expressed in hepatocytes and is induced in non-alcoholic steatohepatitis liver." in: Experimental and molecular pathology, Vol. 95, Issue 2, pp. 199-205, (2013) (PubMed).

Target Details for Chemerin

Target: Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

Alternative Name: Chemerin

Background:

Synonyms: Rarres2, TIG2, tazarotene induced gene 2, Retinoic acid receptor responder protein 2, Tazarotene-induced gene 2 protein, RAR-responsive protein TIG2, Chemerin.

Background: Chemerin, also known as retinoic acid receptor responder 2 (RARRES2) and tazarotene induced gene 2 (TIG2), is a chemoattractant protein for cells expressing G-protein-linked receptor chemokine-like receptor 1 (CMKLR1, ChemR23 or GPCR-DEZ). The protein is synthesized as a secreted precursor, prochemerin (18 kDa), which is poorly active. Upon proteolytic removal of the last six amino acids, prochemerin is converted into mature active chemerin (15-16 kDa) that acts as a strong and highly specific agonist of its receptor CMKLR1. Chemerin is a newly described adipokine with effects on adipocyte differnetiation and metabolism.

Gene ID: 297073

Pathways: Brown Fat Cell Differentiation