NpHR antibody

Catalog No. ABIN1720810

This Rabbit Polyclonal antibody specifically detects NpHR in IF and WB. It exhibits reactivity toward Natronomonas pharaonis (Natronobacterium pharaonis).

Quick Overview for NpHR antibody (ABIN1720810)

Target: NpHR

Reactivity: Natronomonas pharaonis (Natronobacterium pharaonis)

Host: Rabbit

Clonality: Polyclonal

Application: Immunofluorescence (IF), Western Blotting (WB)

Product Details

Purpose: Anti-NpHR | Halorhodopsin

No Cross-Reactivity: Halobacterium salinarum

Cross-Reactivity (Details): Natronomonas pharaonis
Not reactive in: Halobacterium salinarum (HsHR and HsBR)

Predicted Reactivity: Natronomonas pharaonis

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide derived from Natronomonas pharaonis halorhodopsin sequences UniProt: A0A1U7EU03

Application Details

Application Notes: 1 : 200 (IF), 1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: NpHR

Background: Halorhodopsin (HR) is a hyperpolarizing light-driven ion pump from the halophilic archaea bacterium Natronomonas pharaonis. HR uses the energy of yellow light (excitation maximum near 580 nm) to mediate primarily chloride but also bromide, iodide, and nitrate import into the cell against their electrochemical gradients.

Molecular Weight: 30.9 | 30 kDa

UniProt: Q3ITX1