14-3-3 GRF antibody

Catalog No. ABIN1720824

The Rabbit Polyclonal anti-14-3-3 GRF antibody (ABIN1720824) specifically detects 14-3-3 GRF in IP and WB. The antibody is reactive with Aesculus hippocastanum, Arabidopsis halleri, Arabidopsis thaliana, Green bean, Lilium longiflorum (Trumpet lily), Mesembryanthemum crystallinum, Neochloris oleoabundans, Oryza sativa, Panax notoginseng, Picrorhiza kurroa, Vicia faba and Zea mays samples.

Quick Overview for 14-3-3 GRF antibody (ABIN1720824)

Target: 14-3-3 GRF

Reactivity: Aesculus hippocastanum, Arabidopsis halleri, Arabidopsis thaliana, Green bean, Lilium longiflorum (Trumpet lily), Mesembryanthemum crystallinum, Neochloris oleoabundans, Oryza sativa, Panax notoginseng, Picrorhiza kurroa, Vicia faba, Zea mays

Host: Rabbit

Clonality: Polyclonal

Application: Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Purpose: Anti-14-3-3 GRF | General regulatory element

Specificity: TAIR: At4g09000, At4g78300, At4g38480, At4g35160, At4g16050, At4g10450, At4g02520, At4g65430, At4g42590, At4g22300, At4g34760

Cross-Reactivity (Details): Aesculus hippocastanum, Arabidopsis halleri, Arabidopsis thaliana, Lilium longiflorum, Mesembryanthemum crystallinum, Neochloris oloabundans (algae), Oryza sativa, Picrorhiza kurroa, Phaseolus vulgaris, Panax notoginseng, Zea mays, Vicia faba
Not reactive in: This antibody does not bind to 14-3-3-like protein D of Nicotiana tabacum.

Predicted Reactivity: Brassica sp.,Chlamydomonas reinhardtii,Glycine max,Gossypium hirsutum,Nicotiana tabacum,Physcomitrium patens,Pisum sativum,Solanum tuberosum,Triticum aestivum

Purification: Serum

Immunogen: Conserved surface-exposed peptide conjugated to KLH.This sequence is repeated in all 12 ""canonical"" members of the Arabidopsis thaliana GRF/14-3-3 family. GRF1 GF14 chi UniProt: F4JJ94

Application Details

Application Notes: 1: 2000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: 14-3-3 GRF

Background:

Background: GRF (General regulatory factor 2) or 14.3.3s are 30 kDa proteins involved in protein interactions with target proteins containing phosphorylated target sites. Functions of 14.3.3s include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system). A variety of apparently unrelated biological activities, including a role in development and signal transduction - brassinosteroid mediated signaling pathway.

Additional information: This antibody is recognizing recombiant GRF of Lilium longiflorum Lil1433_0 accession: AF191746, Lil1433_2, accession: EF397608 and recombinant GRF1,2,3,5 and 6 of Arabidopsis thaliana: GRF1 14-3-3 chi (At4g09000.1), GRF2 14-3-3 omega (At1g78300.1), GRF3 14-3-3 psi (At5g38480.1), GRF5 14-3-3 upsilon (At5g16050.1), GRF6 14-3-3 lambda (At5g10450.2), GRF8 14-3-3 kappa (At5g65430.1), GRF11 14-3-3 omicron (At1g34760.1).There is also very weak reaction to Physcomitrella patens Pp14-3-3 Pp1s 73_133V6 (closest homolog to AtGRF6) and Chlamydomonas reinhardtii Cr 14-3-3 Cre 12.g559250 (closest homolog to AtGRF6).

Molecular Weight: 20-28 kDa (depending upon an isoform)

Gene ID: 818859, 831462

UniProt: F4JJ94, Q01525, F4KBI7, F4HWQ5, P42645, Q96300, F4KHY7, F4IP55, F4I1C1, Q9S9Z8, Q9C5W6