LexA antibody

Catalog No. ABIN1720850

The Rabbit Polyclonal anti-LexA antibody is suitable to detect LexA in samples from Synechocystis. It has been validated for WB.

Quick Overview for LexA antibody (ABIN1720850)

Target: LexA

Reactivity: Synechocystis

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Purpose: Anti-LexA | SOS function regulatory protein

Cross-Reactivity (Details): Synechocystis sp. PCC 6803 (gene: sll1626, protein: Sll1626)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Cyanobacteria

Purification: Serum

Immunogen: LexA from Anabaena sp. strain PCC 7120 with added His-tag

Application Details

Application Notes: 1 : 16 000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: LexA

Background:

Background: LexA (EC 3.4.21.88) is a DNA binding protein, is associated with SOS response of cyanobacteria as well as other regulatory networs. Has been shown to directly regulate genes involved in carbon assimilation or controlled by carbon availability. In Synechocystis PCC 6803 LexA is not localised to the thylakoid membranes but to the inner region of the cytoplasm

Additional information: Antibody can be used as a marker of cytoplasm but also, most likely, a marker for DNA localisation

Molecular Weight: 26 kDa (for a monomer/subunit)