GIT1 antibody (C-Term)

Catalog No. ABIN1721531

The Mouse Monoclonal anti-GIT1 antibody has been validated for WB. It is suitable to detect GIT1 in samples from Human, Rat and Mouse.

Quick Overview for GIT1 antibody (C-Term) (ABIN1721531)

Target: GIT1

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GIT1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: S39B-8

Product Details anti-GIT1 Antibody

Binding Specificity: AA 375-770, C-Term

Specificity: Reacts with human GIT1. Cross-reacts with mouse and rat protein.

Purification: Purified (protein G)

Immunogen: Fusion protein corresponding to aa 375-770 (C-terminus) of rat GIT1 (accession no.Q9Z272).

Isotype: IgG1

Application Details

Application Notes: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
WB(1 μg/mL)

Positive control: Rat brain lysate.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, glycerol 50 % .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute in PBS or medium that is identical to that used in the assay system.

Storage: -20 °C

Storage Comment: Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GIT1

Target: GIT1

Alternative Name: GIT1

Background: G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains for small GTPases, kinases, the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and it regulates cell protrusion formation and cell migration.

ARF GTPase-activating protein GIT1, ARF GAP GIT1 Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1 G protein-coupled receptor kinase-interactor 1 GRK-interacting protein 1 Gene name : GIT1

Gene ID: 28964

UniProt: Q9Y2X7