GRP78 antibody

Catalog No. ABIN1721537

The Mouse Monoclonal anti-GRP78 antibody has been validated for WB and ELISA. It is suitable to detect GRP78 in samples from Human, Rat, Mouse, Frog, Xenopus laevis and Mammalian.

Quick Overview for GRP78 antibody (ABIN1721537)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Human, Rat, Mouse, Frog, Xenopus laevis, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 6H4-2G7

Product Details anti-GRP78 Antibody

Specificity: Reacts with human GRP78. Cross-reacts with mouse, rat, bovine, hamster, rabbit, monkey, xenopus and fungi (Cryphonectria parasitica) protein.

Purification: Purified (protein G)

Immunogen: His-tagged human GRP78 (accession no. NP_001156906.1)

Isotype: IgG1

Application Details

Application Notes: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
WB(0.5 - 1.0 μg/mL)

Positive control: HeLa cell

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2 mg/mL

Buffer: PBS, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute in PBS or medium which is identical to that used in the assay system.

Storage: -20 °C

Storage Comment: For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 months from date of receipt.

Expiry Date: 6 months

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: GRP78

Background: Categorized as stress response proteins, BiPs play an important role in folding and assembly of nascent proteins and in scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5- nontranslated region of BiP mRNA. BiP IRES activity increases when cells are heat-stressed. GRP78 is also important in maintenance of cell homeostasis and the prevention of apoptosis. Levels of GRP78 protein are reduced in brains of Alzheimer's disease patients.

78 kDa glucose-regulated protein, GRP-78 Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Heat shock 70 kDa protein 5 Immunoglobulin heavy chain-binding protein, BiP Gene name: HSPA5, GRP78

Gene ID: 3309

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling