Hsc70 antibody

Catalog No. ABIN1721551

This Mouse Monoclonal antibody specifically detects Hsc70 in WB, IHC, ELISA and IP. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Hsc70 antibody (ABIN1721551)

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hsc70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Clone: 1F2-H5

Product Details anti-Hsc70 Antibody

Specificity: Reacts with human Hsc70 (Hsp73). Cross-reacts with mouse and rat protein.

Purification: Purified (protein G)

Immunogen: Human Hsc70

Isotype: IgG2a

Application Details

Application Notes: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
WB1 μg/mL ELISA1-10 μg/mL IHC1-10 μg/mL IP1-10 μg/mL

Positive control: HeLa cells

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.4

Handling Advice: Dilute in PBS or medium which is identical to that used in the assay system.

Storage: 4 °C/-20 °C

Storage Comment: Short term storage at+4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 months from date of receipt.

Expiry Date: 6 months

Target Details for Hsc70

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name: Hsc70 (Hsp73)

Background: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes.

Heat shock cognate 71 kDa protein Heat shock 70 kDa protein 8 Lipopolysaccharide-associated protein 1, LAP-1, LPS-associated protein 1 Gene name: HSPA8, HSC70, HSP73, HSPA10

Gene ID: 3312

UniProt: P11142