Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Clone
7C5
Specificity
Reacts with mouse and hamster sperm Protein sp56. Rects with denatured, nonreduced sp56 but not with denatured, reduced sp56. Do not cross-react with human sperm.
Cross-Reactivity (Details)
Reacts with mouse Sperm Protein sp56. Cross-reacts with hamster protein.
Purification
Purified (protein G)
Immunogen
Mixture of native and formaldehyde-zinc fixed sperm purified through a Percoll gradient.
Isotype
IgG1
Application Notes
Working dilution: Optimal dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS, pH 7.4.
Handling Advice
Dilute in PBS or medium which is identical to that used in the assay system.
Storage
-20 °C/-80 °C
Storage Comment
Stable for at least one year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Expiry Date
12 months
Target
Sp56
(Sperm Fertilization Protein 56 (Sp56))
Alternative Name
Sperm Protein Sp56
Background
Zona pellucida sperm-binding protein 3 receptor Sperm fertilization protein 56, sp56 Genename: Zp3r, Sp56