MYST1 antibody

Catalog No. ABIN1724671

This Mouse Monoclonal antibody specifically detects MYST1 in WB, ELISA, IHC and ICC. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for MYST1 antibody (ABIN1724671)

Target: MYST1 (KAT8) (K(lysine) Acetyltransferase 8 (KAT8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYST1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 8C4C4

Product Details anti-MYST1 Antibody

Purpose: MYST1 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human MYST1 expressed in E. Coli.

Isotype: IgG2b

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-MYST1 antibody (ABIN1724671)

Jiang, Gao, Zheng, Yin, Wang, Zhong, Ali, Khan, Hao, Fang, Sun, Xu, Pandita, Jiang, Shi: "MOF influences meiotic expansion of H2AX phosphorylation and spermatogenesis in mice." in: PLoS genetics, Vol. 14, Issue 5, pp. e1007300, (2018) (PubMed).

Akhtar, Becker: "The histone H4 acetyltransferase MOF uses a C2HC zinc finger for substrate recognition." in: EMBO reports, Vol. 2, Issue 2, pp. 113-8, (2001) (PubMed).

Neal, Pannuti, Smith, Lucchesi: "A new human member of the MYST family of histone acetyl transferases with high sequence similarity to Drosophila MOF." in: Biochimica et biophysica acta, Vol. 1490, Issue 1-2, pp. 170-4, (2000) (PubMed).

Sterner, Berger: "Acetylation of histones and transcription-related factors." in: Microbiology and molecular biology reviews : MMBR, Vol. 64, Issue 2, pp. 435-59, (2000) (PubMed).

Target Details for MYST1

Target: MYST1 (KAT8) (K(lysine) Acetyltransferase 8 (KAT8))

Alternative Name: MYST1

Background: MYST1 (MYST histone acetyltransferase 1, MOF) belongs to the MYST family of histone acetyltransferases, which are employed in the cell to bring about transcriptional regulation. The MYST family includes MYST1, is named for the founding members MOZ, yeast YBF2 and SAS2, and TIP60. All members of this family contain a MYST region of about 240 amino acids with a canonical acetyl-CoA-binding site and a C2HC-type zinc finger motif. Most MYST proteins also have a chromodomain involved in protein- protein interactions and targeting transcriptional regulators to chromatin. Although MOF is expressed in both males and females, it associates with the X chromosome only in males. MOF contains a zinc-finger domain that is used to contact the globular part of the nucleosome and histone H4. The carboxy terminal domain of human MOF also has histone acetyltransferase activity directed against histones H3 and H2A, a characteristic shared with other MYST family histone

Molecular Weight: 53 kDa

Gene ID: 84148

UniProt: Q9H7Z6