SYK antibody (AA 296-484)

Catalog No. ABIN1724704

This Mouse Monoclonal antibody specifically detects SYK in WB and ELISA. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for SYK antibody (AA 296-484) (ABIN1724704)

Target: SYK (Spleen tyrosine Kinase (SYK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SYK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 8C1A3

Product Details anti-SYK Antibody

Binding Specificity: AA 296-484

Purpose: SYK Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of SYK (aa296-484) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-SYK antibody (ABIN1724704)

Abbal, Lambelet, Bertaggia, Gerbex, Martinez, Arcaro, Schapira, Spertini: "Lipid raft adhesion receptors and Syk regulate selectin-dependent rolling under flow conditions." in: Blood, Vol. 108, Issue 10, pp. 3352-9, (2006) (PubMed).

Kunze, Wendt, Schlott: "Promoter hypermethylation of the 14-3-3 sigma, SYK and CAGE-1 genes is related to the various phenotypes of urinary bladder carcinomas and associated with progression of transitional cell carcinomas." in: International journal of molecular medicine, Vol. 18, Issue 4, pp. 547-57, (2006) (PubMed).

Wossning, Herzog, Köhler, Meixlsperger, Kulathu, Mittler, Abe, Fuchs, Borkhardt, Jumaa: "Deregulated Syk inhibits differentiation and induces growth factor-independent proliferation of pre-B cells." in: The Journal of experimental medicine, Vol. 203, Issue 13, pp. 2829-40, (2006) (PubMed).

Target Details for SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Alternative Name: SYK

Background: SYK: spleen tyrosine kinase. Syk (72 kDa) is a non-receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.

UniProt: P43405

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling