MBP Tag antibody

Catalog No. ABIN1724707

This Mouse Monoclonal antibody specifically detects in WB, ELISA, IP, ICC, IHC and FACS. It exhibits reactivity toward Tagand has been mentioned in 1 publication.

Quick Overview for MBP Tag antibody (ABIN1724707)

Target: MBP Tag

Reactivity: Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 3G1A3

Product Details

Purpose: MBP Antibody

Purification: Ascitic fluid

Immunogen: Recombinant fusion protein with Maltose binding protein tag.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/100 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References

Chuang, Hsu, Hsu, Hsu, Syu: "Construction of a tagging system for subcellular localization of proteins encoded by open reading frames." in: Journal of biomedical science, Vol. 8, Issue 2, pp. 170-5, (2001) (PubMed).

Target Details

Target: MBP Tag

Target Type: Tag

Background: Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.