MBP Tag antibody

Catalog No. ABIN126834

Product Details

Target: MBP Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody can be used for the detection of MBP fusion proteins.

Characteristics: Synonyms: MMBP, Maltodextrin-binding protein, malE-Tag

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Immunogen: MBP (Maltose Binding Protein)

Clone: 6-00E-004

Isotype: IgG1

Application Details

Application Notes: Western blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT)

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: MBP Tag

Alternative Name: Maltose Binding Protein Tag / MBP-Tag

Target Type: Tag

Background: Maltose Binding Protein (MBP), the E. coli malE gene product, is widely used as fusion partner for the expression of recombinant proteins.Synonyms: MMBP, Maltodextrin-binding protein, malE-Tag

Molecular Weight: 43 kDa

Gene ID: 948538

UniProt: P0AEX9