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GLUT4 antibody (AA 224-353)

Name: GLUT4 antibody (AA 224-353)
Brand: antibodies-online
SKU: ABIN1724743
Price: 533.5 USD
Availability: InStock

SLC2A4 Reactivity: Human WB, IHC, ELISA, ICC, FACS Host: Mouse Monoclonal 3G10A3 unconjugated

Western blot analysis using SLC2A4 mAb against human SLC2A4 recombinant protein. (GLUT4 antibody (AA 224-353))

Immunohistochemical analysis of paraffin-embedded cardiac muscle tissues using SLC2A4 mouse mAb with DAB staining. (GLUT4 antibody (AA 224-353))

IHC

Western blot analysis using SLC2A4 mouse mAb against NIH3T3 (1), 3T3L1 (2), MCF-7 (4) cell lysate and Mouse heart (3) tissue lysate. (GLUT4 antibody (AA 224-353))

Western blot analysis using SLC2A4 mAb against HEK293 (1) and SLC2A4 (AA: 224-353)-hIgGFc transfected HEK293 (2) cell lysate. (GLUT4 antibody (AA 224-353))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (GLUT4 antibody (AA 224-353))

ELISA

Flow cytometric analysis of HeLa cells using SLC2A4 mouse mAb (green) and negative control (purple). (GLUT4 antibody (AA 224-353))

FACS

Immunofluorescence analysis of HepG2 cells using SLC2A4 mouse mAb (green). (GLUT4 antibody (AA 224-353))

Immunofluorescence analysis of HeLa cells using SLC2A4 mouse mAb (green). (GLUT4 antibody (AA 224-353))

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SLC2A4 mouse mAb with DAB staining. (GLUT4 antibody (AA 224-353))

IHC

9 images

(1 reference)

Catalog No. ABIN1724743

Datasheet as PDF

Target See all GLUT4 (SLC2A4) Antibodies

GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))
Binding Specificity
8

6

6

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 224-353
Reactivity
67

49

34

7

6

4

4

3

3

3

2

1

1

1
Human
Host
55

18

2
Mouse
Compatible Secondaries
Clonality
57

18
Monoclonal
Conjugate
45

4

3

3

3

3

3

3

3

2

1

1

1
This GLUT4 antibody is un-conjugated
Application
46

28

27

14

14

7

7

5

5

3

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose

SLC2A4 Antibody

Purification

Ascitic fluid

Immunogen

Purified recombinant fragment of human SLC2A4 (AA: 224-353 ) expressed in E. Coli.

Clone

3G10A3

Isotype

IgG2b
anti-Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4) (C-Term) antibody
SLC2A4 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4) (AA 333-509) antibody
SLC2A4 Reactivity: Human, Mouse, Rat WB, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4) (AA 401-509) antibody
SLC2A4 Reactivity: Human, Mouse, Rat, Pig, Goat, Sheep WB, ELISA, IHC (p), IHC (fro), FACS, IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4) (AA 224-353) antibody
SLC2A4 Reactivity: Human, Mouse WB, IHC, ELISA, IF/ICC, FACS Host: Mouse Monoclonal unconjugated

anti-Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4) (AA 223-287) antibody
SLC2A4 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4) (N-Term) antibody
SLC2A4 Reactivity: Human, Mouse, Rat, Rabbit, Cow, Goat, Horse, Dog, Guinea Pig, Zebrafish (Danio rerio) WB Host: Rabbit Polyclonal unconjugated

anti-Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4) (AA 228-292) antibody
SLC2A4 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4) (C-Term) antibody
Verified SLC2A4 Reactivity: Human, Mouse WB, IHC, ELISA Host: Goat Polyclonal unconjugated

anti-Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4) (AA 467-509) antibody
SLC2A4 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2A4 unconjugated

anti-Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4) (AA 467-509) antibody
SLC2A4 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 4A1 unconjugated

Application Notes

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/200 - 1/1000

Restrictions

For Research Use only
Format

Liquid

Buffer

Ascitic fluid containing 0.03 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Sano, Peck, Kettenbach, Gerber, Lienhard: "Insulin-stimulated GLUT4 protein translocation in adipocytes requires the Rab10 guanine nucleotide exchange factor Dennd4C." in: The Journal of biological chemistry, Vol. 286, Issue 19, pp. 16541-5, (2011) (PubMed).
Target

GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Alternative Name

SLC2A4 (SLC2A4 Products)

Background

Description: This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).

Aliases: GLUT4

Molecular Weight

54.8kDa

Gene ID

6517

HGNC

6517

UniProt

P14672

Pathways

AMPK Signaling, Carbohydrate Homeostasis, Proton Transport, Brown Fat Cell Differentiation, Warburg Effect