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Nucleostemin antibody (AA 1-226)

Name: Nucleostemin antibody (AA 1-226)
Brand: antibodies-online
SKU: ABIN1724770
Price: 533.5 USD
Availability: InStock

This Mouse Monoclonal antibody specifically detects Nucleostemin in WB, ELISA and FACS. It exhibits reactivity toward Human and Mouseand has been mentioned in 1 publication.

Western blot analysis using GNL3 mAb against HEK293 (1) and GNL3 (AA: 1-226)-hIgGFc transfected HEK293 (2) cell lysate. (Nucleostemin antibody (AA 1-226))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (Nucleostemin antibody (AA 1-226))

ELISA

Flow cytometric analysis of Jurkat cells using GNL3 mouse mAb (green) and negative control (purple). (Nucleostemin antibody (AA 1-226))

FACS

Western blot analysis using GNL3 mouse mAb against NIH3T3 (1) and PC-3 (2) cell lysate. (Nucleostemin antibody (AA 1-226))

Western blot analysis using GNL3 mAb against human GNL3 recombinant protein. (Nucleostemin antibody (AA 1-226))

5 images

(1 reference)

Catalog No. ABIN1724770

Quick Overview for Nucleostemin antibody (AA 1-226) (ABIN1724770)

Target

See all Nucleostemin (GNL3) Antibodies

Nucleostemin (GNL3) (Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3))

Reactivity

Human, Mouse

Host

Mouse

Clonality

Monoclonal

Conjugate

This Nucleostemin antibody is un-conjugated

Application

Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Clone

2C8D5

Binding Specificity
15

9

8

4

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 1-226

Purpose

GNL3 Antibody

Purification

Purified antibody

Immunogen

Purified recombinant fragment of human GNL3 (AA: 1-226) expressed in E. Coli.

Isotype

IgG1
anti-Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3) (AA 1-226) antibody
GNL3 Reactivity: Human ELISA Host: Mouse Monoclonal 2C8D5 unconjugated

anti-Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3) (AA 1-549) antibody
GNL3 Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated

anti-Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3) (AA 1-226) antibody
GNL3 Reactivity: Human, Mouse WB, ELISA, FACS Host: Mouse Monoclonal unconjugated

anti-Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3) (AA 1-240) antibody
GNL3 Reactivity: Human WB, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3) antibody
GNL3 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3) (AA 328-427) antibody
GNL3 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 1A1 unconjugated

anti-Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3) (AA 91-124), (N-Term) antibody
GNL3 Reactivity: Human WB, IF Host: Rabbit Polyclonal RB51601 unconjugated

anti-Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3) (AA 442-471), (C-Term) antibody
GNL3 Reactivity: Mouse WB Host: Rabbit Polyclonal RB40720 unconjugated

anti-Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3) (AA 458-487), (C-Term) antibody
GNL3 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB13446 unconjugated

anti-Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3) antibody
GNL3 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1/10000
FCM: 1/200 - 1/400

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Meng, Hsu, Tsai: "GNL3L depletion destabilizes MDM2 and induces p53-dependent G2/M arrest." in: Oncogene, Vol. 30, Issue 14, pp. 1716-26, (2011) (PubMed).
Target

Nucleostemin (GNL3) (Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3))

Alternative Name

GNL3

Background

The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene.

Molecular Weight

62 kDa

Gene ID

26354

UniProt

Q9BVP2