AIF antibody (C-Term)

Catalog No. ABIN1731618

Product Details anti-AIF Antibody

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Binding Specificity: AA 593-606, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIF antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Specificity: Reacts with the C-terminus part of human AIF. Cross-reacts with mouse and rat protein.

Purification: Purified

Immunogen: Peptide corresponding to aa 593- 606 at the C-terminus of human AIF. This sequence is identical to those of mouse and rat AIF.

Isotype: IgG

Application Details

Application Notes: Working dilution: Optimal dilutions should be determined by the end user.
The following are guidelines only:
ICC5 μg/mL WB0.5 - 2 μg/mL

Positive control: K562 cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute in PBS or medium which is identical to that used in the assay system.

Storage: -20 °C

Storage Comment: Store in aliquots at -20°C. Antibodies are guaranteed for 6 months from date of receipt.

Expiry Date: 6 months

Target Details for AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: Apoptosis-Inducing Factor, AIF (AIFM1 Products)

Background: A novel protein that causes chromatin condensation and DNA fragmentation has been designated Apoptosis Inducing Factor (AIF). AIF localizes in mitochondria and translocates to the nucleus when apoptosis is induced. This event is followed by the release of chytochrome c and caspase-9 from mitochondria. AIF is highly conserved between human and mouse and widely expressed in different cell types and tissues.

Apoptosis-inducing factor 1, mitochondrial Programmed cell death protein 8 Gene name: AIFM1, AIF, PDCD8

Gene ID: 9131

UniProt: O95831

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect