AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1)

Details for Product anti-AIFM1 Antibody No. ABIN968950
Target
Reactivity
Human, Monkey, Mouse, Rat
123
71
70
24
14
13
9
8
8
7
5
4
4
2
1
1
Host
Mouse
112
11
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This AIFM1 antibody is un-conjugated
7
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
99
47
40
30
29
18
13
11
9
8
5
2
2
1
1
Options
Immunogen Purified recombinant fragment of human AIF expressed in E. Coli.
Clone 4-00E-07
Isotype IgG2b
Purification Ascites
Target
Alternative Name AIF (AIFM1 Antibody Abstract)
Background This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10
Molecular Weight 67 kDa
Gene ID 9131
HGNC 9131
Pathways Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage
Images
Image no. 1 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) antibody (ABIN968950) Figure 5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tis...
Image no. 2 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) antibody (ABIN968950) Figure 6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissu...
Image no. 3 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) antibody (ABIN968950) Figure 7: Immunohistochemical analysis of paraffin-embedded human lung cancer tissues...
Image no. 4 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) antibody (ABIN968950) Figure 8: Western blot analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), ...
Image no. 5 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) antibody (ABIN968950) Figure 2: Flow cytometric analysis of HepG2 cells using AIF mouse mAb (blue) and nega...
Image no. 6 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) antibody (ABIN968950) Figure 3: Immunofluorescence analysis of NIH/3T3 cells using AIF mouse mAb (green) . ...
Image no. 7 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) antibody (ABIN968950) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Image no. 8 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) antibody (ABIN968950) Figure 9: Western blot analysis using AIF mAb against human AIF (AA: 1-261) recombina...
Image no. 9 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) antibody (ABIN968950) Figure 4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissu...
Product cited in: Son, Jang, Heo, Chung, Choi, Lee: "Apoptosis-inducing factor plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells." in: Apoptosis : an international journal on programmed cell death, Vol. 14, Issue 6, pp. 796-808, 2009 (PubMed).