AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (N-Term)

Details for Product anti-AIFM1 Antibody No. ABIN2855383
Target
Binding Specificity
N-Term
18
15
11
9
5
5
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
123
72
71
24
14
13
9
9
8
7
5
4
4
2
1
1
Host
Rabbit
111
12
2
2
Clonality
Polyclonal
Conjugate
This AIFM1 antibody is un-conjugated
7
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
99
48
41
29
28
18
13
11
10
8
5
2
2
1
1
Options
Immunogen Recombinant protein encompassing a sequence within the N-terminus region of human AIF. The exact sequence is proprietary.
Isotype IgG
Characteristics Rabbit Polyclonal antibody to AIF (apoptosis-inducing factor, mitochondrion-associated, 1)
AIF antibody [N1N2], N-term
Purification Purified by antigen-affinity chromatography.
Target
Alternative Name AIF (AIFM1 Antibody Abstract)
Background This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Several alternative transcripts encoding different isoforms have been identified for this gene.

Cellular Localization: Mitochondrion intermembrane space , Nucleus
Molecular Weight 67 kDa
Gene ID 9131
Pathways Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , HeLa , HepG2 , A375

Restrictions For Research Use only
Format Liquid
Concentration 0.72 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Images
Image no. 1 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (N-Term) antibody (ABIN2855383) IHC-P Image Immunohistochemical analysis of paraffin-embedded human lung cancer, usin...
Image no. 2 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (N-Term) antibody (ABIN2855383) ICC/IF Image AIF antibody [N1N2], N-term detects AIFM1 protein at Mitochondria by imm...
Image no. 3 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (N-Term) antibody (ABIN2855383) WB Image AIF antibody [N1N2], N-term detects AIFM1 protein by Western blot analysis. ...
Image no. 4 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (N-Term) antibody (ABIN2855383) IHC-P Image Immunohistochemical analysis of paraffin-embedded human gastric cancer, u...