AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (C-Term)

Details for Product anti-AIFM1 Antibody No. ABIN2630015
Target
Binding Specificity
C-Term
19
15
10
9
5
5
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse, Pig, Rat
123
71
70
23
14
13
9
9
8
7
5
4
4
2
1
1
Host
Goat
112
12
2
1
Clonality
Polyclonal
Conjugate
This AIFM1 antibody is un-conjugated
7
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
Application
ELISA, Immunofluorescence (IF), Western Blotting (WB)
99
47
41
30
28
19
13
11
10
8
5
2
2
1
1
Options
Purpose AIFM1
Immunogen Peptide with sequence C-NEVAKLFNIHED, from the C Terminus of the protein sequence
Blocking Peptide Blocking peptide for this product available: ABIN2815154
Sequence NEVAKLFNIH ED
Isotype IgG
Specificity This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).
Predicted Reactivity Human, Mouse, Rat, Dog, Pig, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Target
Alternative Name AIFM1 (AIFM1 Antibody Abstract)
Background AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor
Gene ID 9131
NCBI Accession NP_004199, NP_665811, NP_665812, NP_001124318
Pathways Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration
Application Notes

DS WB Results: Approx 70 kDa band observed in lysates of cell line Jurkat and NIH3T3, and in Mouse and Rat Heart and Kidney lysates. Approx. 65 kDa observed in Pig Heart lysates (calculated MW of 66.9 kDa according to Human NP_004199.1 and 66.8 kDa according to Mouse NP_036149.1, Rat NP_112646.1 and Pig NP_001284561.1). Recommended concentration: 0.01-0.1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment

Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Images
Image no. 1 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2630015) ABIN2630015 (0.1µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer) Detect...
Image no. 2 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2630015) ABIN2630015-P1 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, perm...
Image no. 3 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2630015) ABIN2630015-P1 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, perm...
Image no. 4 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2630015) ABIN2630015 (0.01µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Dete...
Image no. 5 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2630015) ABIN2630015 (0.01µg/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35µg...
Image no. 6 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2630015) ABIN2630015 (0.03µg/ml) staining of Mouse (A) and Rat (B) Kidney lysate (35µg protein...