Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (C-Term) Primary Antibody

AIFM1 Reactivity: Human, Mouse, Rat, Pig ELISA, IF, WB Host: Goat Polyclonal unconjugated
Catalog No. ABIN2630015
$481.84
Plus shipping costs $45.00
100 μg
local_shipping Shipping to: United States
Delivery in 6 to 7 Business Days
  • Target
    AIFM1
    Binding Specificity
    C-Term
    • 20
    • 15
    • 10
    • 10
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Reactivity
    Human, Mouse, Rat, Pig
    • 130
    • 72
    • 69
    • 23
    • 14
    • 13
    • 9
    • 9
    • 8
    • 7
    • 5
    • 4
    • 4
    • 2
    • 1
    • 1
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    This AIFM1 antibody is un-conjugated
    • 82
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Application
    ELISA, Immunofluorescence (IF), Western Blotting (WB)
    • 99
    • 44
    • 41
    • 30
    • 28
    • 16
    • 13
    • 11
    • 11
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    Purpose
    AIFM1
    Sequence
    NEVAKLFNIH ED
    Specificity
    This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).
    Predicted Reactivity
    Human, Mouse, Rat, Dog, Pig, Cow
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-NEVAKLFNIHED, from the C Terminus of the protein sequence
    Isotype
    IgG
  • Application Notes

    DS WB Results: Approx 70 kDa band observed in lysates of cell line Jurkat and NIH3T3, and in Mouse and Rat Heart and Kidney lysates. Approx. 65 kDa observed in Pig Heart lysates (calculated MW of 66.9 kDa according to Human NP_004199.1 and 66.8 kDa according to Mouse NP_036149.1, Rat NP_112646.1 and Pig NP_001284561.1). Recommended concentration: 0.01-0.1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    AIFM1
    Alternative Name
    AIFM1 (AIFM1 Antibody Abstract)
    Synonyms
    AIF, CMTX4, COWCK, COXPD6, PDCD8, CG7263, DmAIF, Dmel\\CG7263, GB16024, DDBDRAFT_0187853, DDBDRAFT_0191137, DDB_0187853, DDB_0191137, aif, pdcd8, AIFM1, PCD8, AIFsh2, Hq, Pdcd8, mAIF, Aif, zgc:91994, apoptosis inducing factor mitochondria associated 1, allograft inflammatory factor 1, Apoptosis inducing factor, apoptosis-inducing factor 1, mitochondrial, apoptosis inducing factor, apoptosis inducing factor, mitochondria associated 1, apoptosis-inducing factor, mitochondrion-associated, 1, apoptosis-inducing factor, mitochondrion-associated 1, AIFM1, AIF1, AIF, LOC412212, aif, aifm1, Aifm1
    Background
    AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor
    Gene ID
    9131
    NCBI Accession
    NP_004199, NP_665811, NP_665812, NP_001124318
    Pathways
    Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration
You are here:
help Support