AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (C-Term) Primary Antibody
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- Target
- AIFM1
- Binding Specificity
-
C-Term
- Reactivity
-
Human, Mouse, Rat, Pig
- Host
- Goat
- Clonality
- Polyclonal
- Conjugate
-
This AIFM1 antibody is un-conjugated
- Application
-
ELISA, Immunofluorescence (IF), Western Blotting (WB)
- Purpose
- AIFM1
- Sequence
- NEVAKLFNIH ED
- Specificity
- This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).
- Predicted Reactivity
- Human, Mouse, Rat, Dog, Pig, Cow
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-NEVAKLFNIHED, from the C Terminus of the protein sequence
- Isotype
- IgG
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-
- Application Notes
-
DS WB Results: Approx 70 kDa band observed in lysates of cell line Jurkat and NIH3T3, and in Mouse and Rat Heart and Kidney lysates. Approx. 65 kDa observed in Pig Heart lysates (calculated MW of 66.9 kDa according to Human NP_004199.1 and 66.8 kDa according to Mouse NP_036149.1, Rat NP_112646.1 and Pig NP_001284561.1). Recommended concentration: 0.01-0.1 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:128000.
- Comment
-
Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and U2OS cells. Recommended concentration: 10μg/ml.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- Target
- AIFM1
- Alternative Name
- AIFM1 (AIFM1 Antibody Abstract)
- Synonyms
- AIF, CMTX4, COWCK, COXPD6, PDCD8, CG7263, DmAIF, Dmel\\CG7263, GB16024, DDBDRAFT_0187853, DDBDRAFT_0191137, DDB_0187853, DDB_0191137, aif, pdcd8, AIFM1, PCD8, AIFsh2, Hq, Pdcd8, mAIF, Aif, zgc:91994, apoptosis inducing factor mitochondria associated 1, allograft inflammatory factor 1, Apoptosis inducing factor, apoptosis-inducing factor 1, mitochondrial, apoptosis inducing factor, apoptosis inducing factor, mitochondria associated 1, apoptosis-inducing factor, mitochondrion-associated, 1, apoptosis-inducing factor, mitochondrion-associated 1, AIFM1, AIF1, AIF, LOC412212, aif, aifm1, Aifm1
- Background
- AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor
- Gene ID
- 9131
- NCBI Accession
- NP_004199, NP_665811, NP_665812, NP_001124318
- Pathways
- Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration