AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (C-Term)

Catalog No. ABIN2630015

Product Details anti-AIFM1 Antibody

Target: AIFM1

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Binding Specificity: C-Term

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• N-Term 20
• AA 130-180 15
• AA 70-98 10
• C-Term 10
• AA 1-613 5
• AA 517-531 5
• AA 593-606 5
• AA 103-612 4
• Middle Region 3
• AA 121-613 2
• AA 151-169 2
• AA 582-613 2
• AA 596-613 2
• AA 100-150 1
• AA 103-322 1
• AA 109-122 1
• AA 151-180 1
• AA 183-195 1
• AA 211-260 1
• AA 30-110 1
• AA 420-529 1
• AA 51-100 1
• AA 563-613 1
• AA 61-110 1
• AA 81-130 1
• Intermediate Domain 1
• Internal Region 1
• Isoform 3 1
• Isoform2 1
• Tyr85 1

Reactivity: Human, Mouse, Rat, Pig

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• Human 130
• Mouse 72
• Rat 69
• Pig 23
• Cow 14
• Dog 13
• Horse 9
• Monkey 9
• Sheep 8
• Rabbit 7
• Hamster 5
• Bat 4
• Zebrafish (Danio rerio) 4
• Chicken 2
• Gibbon 1
• Guinea Pig 1

Host: Goat

Clonality: Polyclonal

Conjugate: This AIFM1 antibody is un-conjugated

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• Un-conjugated 82
• Biotin 7
• Alexa Fluor 488 4
• Alexa Fluor 555 4
• Alexa Fluor 594 4
• Alexa Fluor 647 4
• Alexa Fluor 680 4
• Alexa Fluor 750 4
• FITC 4
• HRP 4
• APC 2
• Alkaline Phosphatase (AP) 2
• PE 2
• Alexa Fluor 350 1
• Cy3 1
• Cy5 1
• Cy5.5 1
• Cy7 1

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

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• Western Blotting (WB) 99
• ELISA 44
• Immunohistochemistry (IHC) 41
• Immunofluorescence (IF) 30
• Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28
• Immunocytochemistry (ICC) 16
• Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13
• Flow Cytometry (FACS) 11
• Immunoprecipitation (IP) 11
• Enzyme Immunoassay (EIA) 5
• Immunohistochemistry (Frozen Sections) (IHC (fro)) 5
• Immunochromatography (IC) 2
• Immunofluorescence (fixed cells) (IF/ICC) 2
• Fluorescence Microscopy (FM) 1
• Immunofluorescence (Cultured Cells) (IF (cc)) 1

Purpose: AIFM1

Sequence: NEVAKLFNIH ED

Specificity: This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).

Predicted Reactivity: Human, Mouse, Rat, Dog, Pig, Cow

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-NEVAKLFNIHED, from the C Terminus of the protein sequence

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Approx 70 kDa band observed in lysates of cell line Jurkat and NIH3T3, and in Mouse and Rat Heart and Kidney lysates. Approx. 65 kDa observed in Pig Heart lysates (calculated MW of 66.9 kDa according to Human NP_004199.1 and 66.8 kDa according to Mouse NP_036149.1, Rat NP_112646.1 and Pig NP_001284561.1). Recommended concentration: 0.01-0.1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AIFM1

Target: AIFM1

Alternative Name: AIFM1 (AIFM1 Antibody Abstract)

Synonyms: AIF, CMTX4, COWCK, COXPD6, PDCD8, CG7263, DmAIF, Dmel\\CG7263, GB16024, DDBDRAFT_0187853, DDBDRAFT_0191137, DDB_0187853, DDB_0191137, aif, pdcd8, AIFM1, PCD8, AIFsh2, Hq, Pdcd8, mAIF, Aif, zgc:91994, apoptosis inducing factor mitochondria associated 1, allograft inflammatory factor 1, Apoptosis inducing factor, apoptosis-inducing factor 1, mitochondrial, apoptosis inducing factor, apoptosis inducing factor, mitochondria associated 1, apoptosis-inducing factor, mitochondrion-associated, 1, apoptosis-inducing factor, mitochondrion-associated 1, AIFM1, AIF1, AIF, LOC412212, aif, aifm1, Aifm1

Background: AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor

Gene ID: 9131

NCBI Accession: NP_004199, NP_665811, NP_665812, NP_001124318

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration