APAF1 antibody (AA 12-28)

Catalog No. ABIN1731834

This anti-APAF1 antibody is a Rabbit Polyclonal antibody detecting APAF1 in WB, IHC (p), IF and ICC. Suitable for Human and Mouse.

Quick Overview for APAF1 antibody (AA 12-28) (ABIN1731834)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-APAF1 Antibody

Binding Specificity: AA 12-28

Specificity: Reacts with human and mouse Apaf1.

Purification: Purified

Immunogen: Synthetic peptide, corresponding to amino acids 12-28 of Human Apaf 1. The sequence of the immunogenic peptide differs from that of murine Apaf1 by one amino acid.

Isotype: IgG

Application Details

Application Notes: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
IHC(1 - 2 μg/mL) IF (10 μg/mL) WB(1 - 2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute in PBS or medium that is identical to that used in the assay system.

Storage: -20 °C

Storage Comment: Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles.

Expiry Date: 12 months

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: Apaf1

Background: The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis proteaseactivating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.

Apoptotic protease-activating factor 1, APAF-1 Gene name: APAF1, KIAA0413, CED4

Gene ID: 317

UniProt: O14727

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity