ASK1 antibody (AA 1356-1375)

Catalog No. ABIN1731994

This Rabbit Polyclonal antibody specifically detects ASK1 in WB, IF, ICC and IP. It exhibits reactivity toward Human.

Quick Overview for ASK1 antibody (AA 1356-1375) (ABIN1731994)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-ASK1 Antibody

Binding Specificity: AA 1356-1375

Specificity: Reacts with human ASK1.

Purification: Purified

Immunogen: Synthetic peptide corresponding to aa 1356-1375 of human ASK1. This peptide sequence is different from that of mouse in the last two amino acids

Isotype: IgG

Application Details

Application Notes: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
WB(0.5 - 1 μg/mL) ICC(10 μg/mL)

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute in PBS or medium that is identical to that used in the assay system.

Storage: -20 °C

Storage Comment: Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: ASK1

Background: A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activate c-jun N-terminal kinase (JNK) and p36 MAP kinase. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induces apoptotic cell death. A catalytically inactive form of ASK1 inhibits TNF -induced apoptosis. ASK1 is expressed in a variety of human and mouse tissues.

Mitogen-activated protein kinase kinase kinase 5 Apoptosis signal-regulating kinase 1, ASK-1 MAPK/ERK kinase kinase 5, MEK kinase 5, MEKK 5 Gene name: MAP3K5, ASK1, MAPKKK5, MEKK5

Gene ID: 4217

UniProt: Q99683

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response